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Category Archives: Research Planning
Protected: project 2
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Protected: project 2
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Protected: project 2 coding update
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Protected: project 2 notes
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Protected: project 2 coding
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Protected: new project 2
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oligopaint probe making planning
emPCR ready libraries: AbdA AbdB Ubx en-locus 100kb Black 100kb Yellow 100kb Green 100kb Synthesis schemes: Teritaries + Nicking PCR using extended primers = Forward primer (21bp) + Secondary binding site (32 bp) Nick off R primer Denaturing PAGE Gel … Continue reading
Posted in Chromatin, probe and plasmid building, Research Planning
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multicolor by sequential staining
Approach Objective and Adventages Avoid some of the incompatiability issues of protein-DNA staining: Protein stains in previously untreated tissue should always work DNA stains should still work after protein imaging — it’s just the formamide treatment tends to kill the … Continue reading
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Updating Web Notebook: version management
Methods for version management: WP Document Revisions adds a new tab to main side widget (below Pages and Comments), called Documents. Under this tab you can upload files from your computer. These now behave has posts. Instead of editing the … Continue reading
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Tagged coding, website
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Failures update
Fly Genetics Failed knockdown: 2x Mat alpha tub driving any of the TRiP valium 20 or valium 22 PcG target shRNAs. Gives viable F1 and F2s or infertile F2s. No PcG phenotypes. Kill all current crosses Kill all expanding Gal4 … Continue reading
Posted in Chromatin, Research Planning
Tagged chromatin, fly work, Progress Report
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