Category Archives: Research Planning

Levine Progress Reports, planned experiments, research summary

Protected: project 2

Posted on April 21, 2013 by admin

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Protected: project 2

Posted on April 20, 2013 by admin

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Protected: project 2 coding update

Posted on April 19, 2013 by admin

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Protected: project 2 notes

Posted on April 17, 2013 by admin

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Protected: project 2 coding

Posted on April 16, 2013 by admin

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Protected: new project 2

Posted on April 15, 2013 by admin

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oligopaint probe making planning

Posted on April 1, 2013 by admin

emPCR ready libraries: AbdA AbdB Ubx en-locus 100kb Black 100kb Yellow 100kb Green 100kb Synthesis schemes: Teritaries + Nicking PCR using extended primers = Forward primer (21bp) + Secondary binding site (32 bp) Nick off R primer Denaturing PAGE Gel … Continue reading →

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multicolor by sequential staining

Posted on March 13, 2013 by admin

Approach Objective and Adventages Avoid some of the incompatiability issues of protein-DNA staining: Protein stains in previously untreated tissue should always work DNA stains should still work after protein imaging — it’s just the formamide treatment tends to kill the … Continue reading →

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Updating Web Notebook: version management

Posted on March 1, 2013 by admin

Methods for version management: WP Document Revisions adds a new tab to main side widget (below Pages and Comments), called Documents. Under this tab you can upload files from your computer. These now behave has posts. Instead of editing the … Continue reading →

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Failures update

Posted on March 1, 2013 by admin

Fly Genetics Failed knockdown: 2x Mat alpha tub driving any of the TRiP valium 20 or valium 22 PcG target shRNAs. Gives viable F1 and F2s or infertile F2s. No PcG phenotypes. Kill all current crosses Kill all expanding Gal4 … Continue reading →

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