Monthly Archives: March 2013

Friday 03/01/13

8:10 A – 8:10P, 9:30P – 11:00P Confocal helping scan Hazen’s sections Embryo section staining BXC DFISH NO BX-C staining detectable after post-hybe hot-wash (50-60C in waterbath, 25 min). Repeat staining, try short coldwash and then check cells for signal. … Continue reading

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Failures update

Fly Genetics Failed knockdown: 2x Mat alpha tub driving any of the TRiP valium 20 or valium 22 PcG target shRNAs. Gives viable F1 and F2s or infertile F2s. No PcG phenotypes. Kill all current crosses Kill all expanding Gal4 … Continue reading

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Demo Gist-based version management

Protocol: Oligo Paint Synthesis permalink to most recent version. permalink to the version as it stood when this demo was published on 03/21/13. Gist embedded version of date-specific permalink using native Gist embed interpreter for markdown: [easyembed field=”Gist1″]

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demo: version managed pdf protocol

Protocol Qiagen Miniprep (QIAprep) Latest Updated version. Date specific version

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