Monthly Archives: April 2013

Protected: lab meeting 04/15/13

Posted on April 15, 2013 by admin

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Sunday 04/14/13

Posted on April 14, 2013 by admin

5:30 P – 8:00P OligoPaint Analyzing previous gel images Conclusion: something went weird, possibly with original PCR used to seed most of the second round PCRs. Plan Repeat PCRs from scratch Run diagnostic denaturing gels at each step in addition … Continue reading →

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experimental oligopaint PCR 3

Posted on April 14, 2013 by admin

Samples (1 mL scale) y nicked 2x Ya-primer b nicked 1x Ba-primer + 1 x CPPT ubx nicked 1x UBx-adapter-primer + 1x CPPT y PPT: 5 uL YA + 20 uL PPT y PPT control: 5 uL YA + 20 … Continue reading →

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denaturing diagnostic gels

Posted on April 14, 2013 by admin

Gel 1, 5% Agarose, 200 V in sodium-Borax (300 V melts gel). Gel 2, 15% Urea, 120V 2 hrs Gel 3 15% Urea gel, 120 V 50 min in 37C room Gel 4, 10% precast Urea denaturing gel, 120 V, … Continue reading →

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Saturday 04/13/13

Posted on April 13, 2013 by admin

3:00 P – 8:00 P, 9:00 P – 11:00 P Working on review Finalize discussion Touch up figures Touch up figure captions reread once through Send to Stas, Peter, Carl and David for comments OligoPaint troubleshooting Qubit data 10% denaturing … Continue reading →

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qubit data

Posted on April 13, 2013 by admin

concentrations y1 D = 56.1 ug/mL (50*8 uL) = 22.4 ug y1 D F = 82.6 ug/mL (150 uL) = 12.3 ug y10 D = 294 ug/mL in 110*8 uL = 258 ug y10 D F = 96 ug/mL in … Continue reading →

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Reorganizing Protocols

Posted on April 12, 2013 by admin

Goals version managed protocols Protocols page directs to most recent version of the protocol Most recent version of protocol is linked to all previous revisions, and can be diff compared Day-to-day implementation of the protocol permalinks the version as it … Continue reading →

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qubit data

Posted on April 12, 2013 by admin

results prior to extraction y prot contr = too high BP 1/2 PCR = 38 ug/mL 7:20 P, reusing standards, 10.9 BP control = 72.4 ug/mL BP = 35 ug/mL Y-T em 1/2 = 9.16 ug/mL. 7:20P, reusing standards, = … Continue reading →

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Friday 04/12/13

Posted on April 12, 2013 by admin

Web Notebook Updates Switched to Markdown-on-Save plugin instead of WP markdown supports Markdown Plus features like tables etc. Saves original and markdown separately, no corrupting the original entry format because the markdown compiler doesn’t recognize things as a list. Reorganizing … Continue reading →

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Thursday 04/11/13

Posted on April 11, 2013 by admin

Oligo Paint Probe synthesis Test PCRs at 1 mL scale: Assymetric y-PCR, Y-adapter, no Y-L primer + 1uL of EtOH extracted Y PCR product at 85 ng/uL Assymetric b-PCR, B-adapter + 1 uL of EtOH extracted B PCR product at … Continue reading →

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