Monthly Archives: May 2013

Tuesday 05/21/13

Posted on May 21, 2013 by admin

8:30 A – 5:00 P, 7:30 P – 11:45 P Probe making ethanol precipitations Ethanol precipitate B/Y/K/G probes from 2-5 mL scale synthesis (which had poor nicking and extra-large bands). Ethanol precipitate new 4 mL scale B/Y/K/U PCRs with primer-fixed … Continue reading →

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Protected: Project 2

Posted on May 21, 2013 by admin

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Monday 05/20/13

Posted on May 20, 2013 by admin

9:20 A – 7:00P, 9:30P — 12:20 A Lab meeting visiting post-doc candidate: notes Probe making figure out increased library concentrations for large scale PCR for B/Y/K/G libraries set up 5 mL scale PCR for B/Y/K/G using nick-containing primers Setup … Continue reading →

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Group meeting

Posted on May 20, 2013 by admin

Postdoc interview Albert Tsai Observing translation in real-team: tracking prokaryotic translation with single molecule fluorescence. Jody Puglisi (Stanford) Introduction ribosome (20×25 nm) translation: many stochastic and cyclical substeps. Difficult to synchronize large number of possible states. time resolution 10ms. Project … Continue reading →

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Sunday 05/19/13

Posted on May 19, 2013 by admin

10:30 A – 10:00 P, 11:00 P – 12:15 A Probe extraction Previous test gel, suggesting some samples at least have probe: Run denaturing gel at 100 uL scale 95 minutes. Cut out smallest band over 50 bp. sample loading … Continue reading →

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Saturday 05/18/13

Posted on May 18, 2013 by admin

11:00 A – 7:30 P, 8:30 P – 12:30 A Probe making cast 3 15% TBE Urea PAGE gels 0.75mm 15 well cast 1 15% TBE Urea PAGE gel 1.5mm 5 well PAGE gel lanes: ULR ladder 1:2 in TBE, … Continue reading →

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Friday 05/17/13

Posted on May 17, 2013 by admin

10:00 A -7:30P STORM Finish O/N STORM run Recorded 9 200,000 frame movies in very close proximity regions. Last image no signal — focus issue or bleaching? Probe making analyzing gel of test digest Ethanol precipitating and re-supending nicked probe … Continue reading →

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Thursday 05/16/13

Posted on May 16, 2013 by admin

10:00 A – 8:00 P, 9:00P – 12:14 A Feducials Suggestions from Ke Dual-Objective scope has a dual-pass filter to allow green beads to show through Add beads in imaging buffer (no need for washing). Try 625 beads. Use PBS … Continue reading →

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Wednesday 05/15/13

Posted on May 15, 2013 by admin

10:15 A – 7:30P, 8:30P – 12:30 A Probe design ordered new 405 oligo-probes with short attachment ordered unlabeled 30 bp AATAT probes with primary extentions ordered unlabeled 30 bp AATAT probes with double length primary extention to bind 32mer … Continue reading →

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Tuesday 05/4/13

Posted on May 14, 2013 by admin

10:00 A – 5:00 P, 9:00 P — 11:45 P Analysis of signal and background in different hybe conditions Discussion with Brian on temperature results Additional notes not yet sent: Hybe conditions that reduce binding to reduce background seem to … Continue reading →

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