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Monthly Archives: May 2013
Protected: more probe making approaches
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Posted in probe and plasmid building
Tagged images, probe making
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Monday 05/13/13
9:30 A – 7:00 P, 9:35 P – 11:15 P Post-doc candidate interview see notes Gheorghe Chistol (Bustamante Lab) Presentation stuff Touch up slides for post-doc candidate chats Communication Feedback from Chicago: Some support from Stats department Ecology/Evolution finds “math … Continue reading
Posted in Summaries
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Protected: Gheorghe Chistol postdoc interview
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Posted in Lab Meeting
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Sunday 05/12/13
10:00 A — 8:00 P, 9:15 P – 11:30 P OligoPaint Probes new PCR Pool samples: 4 mL Ubx, AbdA and AbdB, 2 mL each B, Y, K and G. cooling down ultra-centrifuge (takes 3 hours, good thing to start … Continue reading
Posted in Summaries
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Saturday 05/11/13
3:00 P – 8:00 P, 9:30 P – 11:15 P Kinetic modeling Discussion with Jeff sent text books (see email) Lent library book (Feller) to Jeff should write text together? OligoPaint Probe making spin down ethanol precipitating samples of probe … Continue reading
Friday 05/10/13
9:00 A – 10:33 P OligoPaint Probe making Spin down crushed gels and aspirate fluid. Attempt to nanodrop. ~200/UV peak, readings probably meaningless. Could Qubit but hopefully these are single-stranded now and shouldn’t read. nanodrop no 260 peak, so reading … Continue reading
Posted in Summaries
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Protected: project 2 update
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Posted in Summaries
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Thursday 05/09/13
9:30 A – 7:30 P, 8:30 P – 12:30 A Literature Interesting paper by Sanchez and Gore on altruists, cheating, and ecological stability in yeast Clear neat article in Nature on being a scientist. Oligo Paint probes Pool O/N PCRs … Continue reading
Posted in Summaries
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Wednesday 05/08/13
9:30 A — Probe making pool digests aliquot out test DNA: 1 uL digest DNA, 3 uL TBE, 1 uL 6x loading dye + 12x gel green, 5 uL formamide with 5% 5M EDTA Pour new 16% urea PAGE gels … Continue reading
Posted in Summaries
Tagged literature, probe making, STORM, STORM analysis
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Protected: Ph-Flag notes
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Posted in Chromatin
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