Monthly Archives: June 2013

Sunday 06/30/13

10:45 A – 8:10 P, 9:00 P – 10:00 P STORM 488 wasn’t bright enough to see single molecules at all last night try with completely fresh glox, some switching events detectable in nucleus. Alexa488 not as bright as Atto. … Continue reading

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Saturday 06/29/13

11:15 A — 10:30 P morning tasks break down O/N STORM run (G + feducials) Transfer data to ProRAID check status of O/N STORM analysis communication Re-contact Asyu about limb buds Write to Li about kb scale Kc HiC data … Continue reading

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Friday 06/28/13

10:00 A – 12:45 A morning tasks break down O/N STORM2 run on B + feducials transfer data to ProRAID Slides for XZ meeting explain Ph project outlook is good should compare to Pc clusters DNA in situs 12 min … Continue reading

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Thursday 06/27/13

10:15 A – 8:30 P Cell culture Fix Kc cells cells have lost adherent properties all washed off. Cells still not attached to new passaged flask either. Probably time to restart this stock from frozen anyway. DNA in situs En … Continue reading

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Wednesday 06/26/13

10:20 A – 12:45 A Literature Xie lab paper on single-molecule imaging of TF binding live Bickmore lab paper on NIPBL affects euchromatin compaction in a cohesin independent manner Ph project Project meeting update. Key next steps: transcriptome and compaction … Continue reading

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Tuesday 06/25/13

10:00 A – 5:30 P, 9:45 P – 12:15 A STORM + Analysis Finish O/N STORM run Copy datafiles to ProRAID # Analyze latest Y and B Kc167 cell datasets Cell culture + Hybes # passage Kc167 cells # plate … Continue reading

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Monday 06/24/13

10:30 A – 6:30 P, 8:30 P – 10:45 P General post-conference paperwork Ph project working on explanation of clustering quantification fig send fig to Ajaz. plan to do Pc or Psc later this week. see post Planning sign up … Continue reading

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Protected: project 2 update: 06/24/13

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Protected: Ph update

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Friday 06/14/13

10:00 A – 7:00 P SDB presentation prep UCSC genome browser refuses to load E4-8 PolII track with certain other tracks working on slides

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