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Monthly Archives: July 2013
Wednesday 07/31/13
9:45 A – 7:30 P, 8:00 P – 10:00 P Discussion discuss bivalent domains with Alex Schier. read Vastenhouw & Scheir review. Schier group lab meeting tomorrow 3:45 STORM finish O/N run of Ph-Wt-flag-cy7, Pc-647 start analysis of en data. … Continue reading
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chromatin paints activator tests
Possible matches: * X-1, cy5-2 * 0-1, cy5-2 * 405-1, cy5-2 * cy5-1, cy5-2 * cy5-1, X-2 Blind-Analysis Round 0 Round 1 R1S1 0-1, cy5-2 R1S3 405-1, cy5-2 R1S4 cy5-1, X-2 R1S5 cy5-1, cy5-2 R1S6 X-1, cy5-2 Round 2 R2S1 … Continue reading
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Tuesday 07/30/13
9:30 A – 5:00 P, 9:00 P- 12:00 P STORM set up O/N Ph-wt Pc-cy7 Modeling Using renewal equations which say fano factor of time is eta^2 of transcript count variability (covN) should go like 1/kf for all N for … Continue reading
Posted in Summaries, Transcription Modeling
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notes from analytical exploration of effects of chain length on mRNA variability
The details of this approach are not valid, since we require reversability to use this form for the variance. excised text from review draft: % Using Sanchez Approach Since only one state in the gestation cycle is transcriptionally competent, all … Continue reading
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Monday 07/29/13
9:45 A – 7:00 P remotely 8:30 P – 1:00 A lab meeting see notes Journal club suggestions from XZ – if it’s not generally relevant, give big picture, not detailed results. Show multiple papers. If everyone can relate it’s … Continue reading
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Protected: project 2, 07/29/13
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Protected: lab meeting 07/29/13
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Sunday 07/28/13
Embryo imaging continue imaging embryo En locus in En-expressing/non-expressing cells Ph-project *label new dk anti-m cy7 Review working on derivation of CoV for total mRNA levels dependence on N Inverse of tri-diagonal matrices: http://www.mat.uc.pt/preprints/ps/p0516.pdf Solving Recurrence Relations: http://www.wikihow.com/Solve-Recurrence-Relations split up … Continue reading
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Protected: project 2, 07/26/13
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Friday 07/26/13
Project 2 see notes Embryo sequential staining checking DNA FISH stain on En-embryos locating all embryos on coverglass using 4x 488-scan. embryos successfully retained through in situ procedure. Both coverslips. yay! Imaging embryos
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