Monthly Archives: May 2014

Protected: project 2: update 05/06/14

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Monday 05/05/14

9:00a – 5:00p Lab Meeting Guest speaker, see post. Multicolor STORM more trouble-shooting observed that 647 images look particularly weak, especially given the brightness of these spots. 15 min of max 405 completely bleaches the 647 spot. (~50K frames). 561 … Continue reading

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lab meeting 05/05/14

Jean-Ju Chung, Spatial Organization of CatSper in Sperm (former collaborator of Sang-He) Clapman lab results now published, to appear soon. background ejaculated sperm not motile outside female environment. Hence ‘not fertile’. what’s the difference? Bicarbonate rich, Albumin. pH changes sharply … Continue reading

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Sunday 05/04/14

10:30a – 10:00p Goals Poster revisions as detailed by XZ. Relaunch Oligo-Array analysis — matlab control script killed by Odyssey. Test new 2-clr STORM stains Questions for RC computing use general queue / infiband to analyze data on Odyssey CPUs … Continue reading

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Protected: Project 2 update: 05/04/14

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Saturday 05/03/14

6:30p – 8:30p STORM setup imaging of E04 high background. Otherwise dyes look very bright, switching looks great.

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Friday 05/02/14

9:00a – 6:30p, 11:45p – 1:45a STORM hard-drive disconnected itself somehow in the wee hours of the morning (must have been around 5 or 6am) reconnected drive, continued collecting data. focus lock gets hella confused at certain positions — full … Continue reading

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Protected: project 2: coding 5/01/14

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Thursday 05/01/14

8:30a — 11:00p OligoArray development writing new batch processing functions to run OligoArray with sbatch on Odyssey. encorporating new functions into matlab-functions. Pulled matlab-storm, matlab-functions, and storm-analysis repositories into my remote folder on Odyssey /n/home05/boettiger/ debugging remote OligoArray launcher. A … Continue reading

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