Tag Archives: cell culture

Thursday 08/22/13

Posted on August 22, 2013 by admin

10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading →

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Thursday 01/17/13

Posted on January 17, 2013 by admin

10:30 A – 7:00P, 8:30P – 9:30P (8:30 – 10:30 workout session) Probe making combine, precipitate and pellet en-647 oligoPAINT probe # Wash, combine, and resupend probe-pellet, # Prep for digestion reaction Cell culture (500 kbp probe test 2) After … Continue reading →

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Wednesday 01/16/13

Posted on January 16, 2013 by admin

9:30 A – 7:30 P  Finish comments on counting comparison for ML # Finish revisions Write to MERCK about Probe Making Precipitate and resuspend RNA-FISH DNP probes for tou, inv, en, Antp and Dfd. Make new RNA-Hybe soln to resuspend … Continue reading →

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Monday 12/17/12

Posted on December 17, 2012 by admin

9:40 A – 8:00P, 9:30-10:30P final prep for lab meeting Give lab meeting presentation 10A-1P Meeting with XZ, ~4P Meeting with Max, discuss MTD knockdown ~4P Snail Missing JPA and highlights for CR paper, upload now Cell reports submission site … Continue reading →

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Friday 12/14/12

Posted on December 14, 2012 by admin

10:15 A – 8:00 P, 9:00P – 11:30P Probe making LC library arrived today IDT primers arrived Cell culture diluted recovering S2R+ culture and Kc160 cells (prior to splitting). Move back to 28C incubator Testing temperature in 28C incubator. S2 … Continue reading →

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Monday 12/10/12

Posted on December 10, 2012 by admin

9:50 A -7:40P, 8:40P – 12:30 A yari lab meeting De-noising: use conventional image as low pass filter to remove non-blinking background Use conventional alignment for drift correction, translate all STORM movies to match multi-color conventional image. Use field of … Continue reading →

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Monday 10/29/12

Posted on October 29, 2012 by admin

10:40 A – 8:15 P lab meeting canceled due to hurricane Sandy contact ML about revisions. Got suggestions for graphical abstract, now implemented.  Waiting for response on text. contact Ting about T7 primers Plan to meet with Brian and Fred (Thursday? … Continue reading →

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Wednesday 10/24/12

Posted on October 24, 2012 by admin

9:15 A – 7:00P, 8:00P – 12:00A RNA FISH + Immuno-stain in cultured cells (developing protocol:) warming up hybe solution for hot washes remove, label and save probes (in yellow -20C dilute probes box, labeled as S2). 3x Hot wash … Continue reading →

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Tuesday 10/23/12

Posted on October 23, 2012 by admin

9:20 A – 6:30P, 8:00P-11:00P (remote) S2 cell DNA-FISH labeling Fixing S2 cells for DFISH (1/2x PBT 5% formaldehyde) cells still attached, looks promising. cells preincubated at 92C for 3min, 60C for 20min, moved to RT probes denatured at 92C … Continue reading →

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