Tag Archives: cell labeling

Tuesday 01/08/13

Posted on January 8, 2013 by admin

10:00A – 10:30P (8:30-10A lab exercise/training) Bistability First draft of deterministic model figure and corresponding text Trying to figure out correct integral to compute an energy surface from the vector field.  Not working so far. Fly work collect virgins: (now … Continue reading →

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Tuesday 12/18/12

Posted on December 18, 2012 by admin

11:00 A -7:30P, 9:15P-9:55P Cell culture S2 cells in chamber wells too overgrown S2 cells in 75 cm^2 plates, in need of passage s2R+ in chamber wells look good for fixation.  Not too spread out despite polyL though. S2R+ in … Continue reading →

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Sunday 12/09/12

Posted on December 9, 2012 by admin

10:20 A – 7:30P, 9:30-11:00P STORM 750 1600 mA = 18 mW 1.45 mW 405 200 mW set 647 Made new Glox 750 not switching well.  Not sure if it’s just low labeling (405 looked okay but not strong). 647 … Continue reading →

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Saturday 12/08/12

Posted on December 8, 2012 by admin

11:00 A – 7:30P, 8:45P – 10:00P Cell staining label with m-HP1a -405,750 and rb-H3K27me3 -488 (top) or rb-H3K27ac -488 (bottom row) Polytene cell staining label with m-HP1a-Cy3b and rb H3K4me3 -488 or rb H3K27me3-488. check staining on confocal some … Continue reading →

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Wednesday 12/05/12

Posted on December 5, 2012 by admin

9:30 A  – 7:40P, 9:00P – 12:00A Confocal dot distances Finish O/N confocal run of Ubx-AbdA data Probe making sent order to LC Sciences for chromatin types On why previous probe might have failed:  over drying? — DNA and RNA … Continue reading →

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Tuesday 12/04/12

Posted on December 4, 2012 by admin

9:40 A – 7:45P, 9:00P – 1:00A Dfd/tub analysis Break down O/N confocal run 3 channels, speed 6, 22 positions, 17.5 finished in 11 hour run.  contact zeiss rep Casey about changing channels between z-stacks rather than every section, should … Continue reading →

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Friday 11/30/12

Posted on November 30, 2012 by admin

9:30A – 10:15 P Fly Work Collect sna[18] x Double balancer virgin and male F1s PM virgin collection STORM2 time. Colenso removing gas laser from STORM2 Mount ultra cryo section embryo for imaging sections not flat or hard to find. … Continue reading →

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Wednesday 11/27/12

Posted on November 28, 2012 by admin

10:15 A — 11:15 PM Polytene cell staining  hot washes in 2x SSCT (45 C in coplin jar in dry oven) check staining — cent probe looks good (rather small foci though) Antibody staining H3K9 did not work.  Should have … Continue reading →

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Friday 11/23/12

Posted on November 23, 2012 by admin

11:00 A – 2:00P, 7:00P – 10:45 P probe making: precipitate, wash, resuspend and freeze probes. Ready to try. Cryo-section staining secondary stain and wash of DFISH coverslips No sections remaining on DFISH coverslips.  Try crosslinking? staining on coverglass does … Continue reading →

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Wednesday 11/22/12

Posted on November 22, 2012 by admin

9:10 A — 8:00P, 10:30P – 12:45 A UltaCryo collect prepped samples 10A – 2P, cutting ultra cryo sections of 1-10hr MTD DFISH-fixed embryos For next time: denser embryo packing in smaller gelatin sections Cryo protect at least 30 min … Continue reading →

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