Tag Archives: chromatin

Tuesday 03/04/14

Posted on March 4, 2014 by admin

8:30a – 9:45a, 2:00p – 11:45p computer stuff Copying files extremely slow. 335 Gb copy from ProBox 1 less than half done computer seems to have taken the night off. Splitdax was on like 115 of 136 at midnight and … Continue reading →

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Monday 02/24/14

Posted on February 24, 2014 by admin

(sick, working from home) Library3 prep continues running primer finding adding more filtering do a direct comparison on final 5 basepairs, reject all perfect matches, keep only unique ones. reran a few times to debug raising T min to 74C … Continue reading →

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Thursday 02/21/14

Posted on February 20, 2014 by admin

5:30p – 8:00p, 9:00p – 10:30p (snowshoeing). Random Walk Polymers Quick reference with the scaling of the self-avoiding RWP (exponent = 3/(2+d), where d is the spatial dimension). Thus 0.6 for 3D SAW polymer More interesting stuff http://arxiv.org/pdf/0711.3679v1.pdf Pivot + … Continue reading →

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Wednesday 02/19/14

Posted on February 19, 2014 by admin

9:45a – 11:45p Goals Today Final prep for meeting with Xioawei add slides showing density STORM analysis launching drift bead analysis for F05 / F06 images and Ph-iFISH data launching split-dax for all un-analyzed datasets on RAID2 and Pro8. running … Continue reading →

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Tuesday 02/18/14

Posted on February 18, 2014 by admin

9:45a – 7:00p, 9:00p – 12:20a STORM Imaging G123 (sample 1 from last staining). Very, very high nuclear background (and strong signal). surprising given the concentration used, but maybe should try 1/10th concentration. STORM analysis Cajal crashed, all jobs got … Continue reading →

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Monday 02/10/14

Posted on February 10, 2014 by admin

9:30a – 11:50p Meetings Group meeting (see notes) Journal club team project meeting Literature interesting perspective: http://www.sciencemag.org/content/343/6171/596.full Pauli’s paper: discovering new developmental signalling peptide (Science) Chromatin Data analysis Analyzed G01-G02 up to image 23 start (re-)analyzing F03. F03 data analyzed … Continue reading →

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Sunday 02/09/14

Posted on February 9, 2014 by admin

10:20a – 9:40p Chromatin project finish analyzing new ANTC data Analyze all remaining complete yellow domains Analyze G01, G02, G03, G04, G01+G02 Progress notes: see protected post

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Saturday 02/08/14

Posted on February 8, 2014 by admin

10:30a – 4:45p, 10:00p-11:50p Chromatin Deep sequencing of libraries finish gel extraction aborted last night due to mini-centrifuge failure. Data analysis Analyze new ANTP data issue: need to fix integrated drift correction, have some weird errors interpolation was not correct … Continue reading →

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Friday 02/07/14

Posted on February 7, 2014 by admin

10:00a – 11:30p PH project Meetings Finalize slides discussion with XZ Add effect on cluster count instead of effect on cluster size. Updates working on figure for the effects on cluster count added cluster counts vs. cluster diameter some further … Continue reading →

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Thursday 02/06/2014

Posted on February 6, 2014 by admin

10:00a – 12:50a Ph Project Meeting with Ajaz, discuss paper revisions. continue preparing slides for discussion with XZ. Meeting notes (4p – 7:30p) To image by Confocal (top priority) Endogenous Ph Endogenous Pc Endogenous Psc (may not work) Ph-Flag Ph-M-Flag … Continue reading →

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