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Tag Archives: coding
Sunday 12/29/13
9:00a – 12:15a Goals Write to Ajaz about new plans Read in _oligo.txt files and analyze probe distributions STORM G1-G2 Start analysis of F12-G01 and F12-G11 data continue work on STORMrender Manual Literature Reading Kieffer-Kwon et al Cell 2013. link … Continue reading
Posted in Summaries
Tagged coding, images, literature, probe making, STORM
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Sunday 12/22/13
9:45a – 11:50p Goals align 750-647 G1 double stains matlab-storm STORMrender update menu options for feducial drift correction to allow finding the bin file of feducial data using the browser (oops, this was already the default for an empty input, … Continue reading
Friday 12/20/13
10:00a – 12:45a STORM imaging need images of 715 beads to align A750 and A647 channels Making new 715 bead slide at 1:20,000. If this looks good we can try a 2-color bead slide as well. trimming the tape so … Continue reading
Wednesday 12/18/13
10:30a – 8:15p, 12:00p- 1:00a Meeting with XZ prepping slides (see notes) Test probes synthesis analyze tomorrow Coding matlab-storm Work with Jeff to restructure startup / installation need to update readme need to update all global variables to new conventions … Continue reading
Friday 12/13/13
10:45a – 12:15a Notebook backed up notebook updated wordpress Z-calibration cross correlation doesn’t improve curve alignment visibly — curves are fundamentally a bit different no spatial structure evident Ph Project reviewing simulation results saved as simulationName_2.eps to file Test new … Continue reading
Posted in Summaries
Tagged chromatin, coding, project2, STORM analysis
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Thursday 12/12/13
10:45a – 11:00p Ph Simulations Troubleshooting extra along bonds. Oops, actually just resulted from plotting the wrong attachment matrix for the given position list. Simulations ran at subsaturating Ph — more total sites than available Ph. Hence there is a … Continue reading
Posted in Summaries
Tagged cell labeling, coding, Ph, troubleshooting
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Protected: project 2 update 12/10/13
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Protected: Saturday 11/30/13
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Posted in Summaries
Tagged cell labeling, coding, STORM, zcal
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Wednesday 11/27/13
Goals Sequencing analysis plots of coverage uniformity per probe set plots of failed probe vs. purity using new indexed method (or maybe just a length cut-off) Ph Project restart simulations Genomics start building library 4 (on Monet) start building library … Continue reading
Sunday 11/24/13
10:00a – 11:00a, 3:00p – 5:00p, 7:00p-12:30p Goals Deep Seq Analysis Analyze distribution along probe region of copy number variation Analyze sequence dependence of copy number variation If a sequence contains a deletion, do we also have that same sequence … Continue reading