Tag Archives: embryo labeling

Friday 11/30/12

Posted on November 30, 2012 by admin

9:30A – 10:15 P Fly Work Collect sna[18] x Double balancer virgin and male F1s PM virgin collection STORM2 time. Colenso removing gas laser from STORM2 Mount ultra cryo section embryo for imaging sections not flat or hard to find. … Continue reading →

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Thursday 11/29/12

Posted on November 29, 2012 by admin

9:20 A — 7:15P, 8:15P – 10:00P Polytene cell Check polytene cell staining.  Faint glow of nuclear laminin visible.  pretty dim, does not have ring shape, see top and bottom (would be easier to distinguish as diffinitive stain by confocal). … Continue reading →

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Wednesday 11/27/12

Posted on November 28, 2012 by admin

10:15 A — 11:15 PM Polytene cell staining  hot washes in 2x SSCT (45 C in coplin jar in dry oven) check staining — cent probe looks good (rather small foci though) Antibody staining H3K9 did not work.  Should have … Continue reading →

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Saturday, 11/24/12

Posted on November 24, 2012 by admin

11:15 A -7:30P, 9:30P – 11:45 P UltraCryo coverslip DFISH Hot washes for DFISH coverslips RT wash check sections on coverglass A.  Be very careful to keep sections covered with buffer. Staining definetely works.  Signal somewhat dimer than expected, but … Continue reading →

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Friday 11/23/12

Posted on November 23, 2012 by admin

11:00 A – 2:00P, 7:00P – 10:45 P probe making: precipitate, wash, resuspend and freeze probes. Ready to try. Cryo-section staining secondary stain and wash of DFISH coverslips No sections remaining on DFISH coverslips.  Try crosslinking? staining on coverglass does … Continue reading →

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Thursday 11/23/12

Posted on November 21, 2012 by admin

10:30 A – 7:30 P Thanksgiving Fly work expand YWs Flip stocks check crosses.  double HbZ not yet emerging.  recombineering crosses looking bleak, we’ll see in anotehr 5-6 days… Probe making New library stats spin down dissolving probe.  Gently Pippett … Continue reading →

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Wednesday 11/14/12

Posted on November 14, 2012 by admin

9:00A – 7:30P, 8:15P – 11:15P Prepping gel (low on Na Borax, and TAE making up new TAE) Test gel: YW postive control AbdB looks great.  YW negative control sim[D] is clean.  Improvised gel extractions not looking good. Try sim[D] … Continue reading →

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Sunday 11/04/12

Posted on November 4, 2012 by admin

11:00 A – 8:00P Cell culture and cell labeling Start washing out direct-labeled primaries from cell-culture plates. Split cells, toss old plates. plate cells on round cover glass in 12 well pates.  See if these adhere any better for FISH-on … Continue reading →

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Saturday 11/03/12

Posted on November 3, 2012 by admin

10:45 A – Writing Write BJ review Drosophila PWMs TF binding motifs FlyFactor Ecoli TF binding + PWMs here (might be something better) Finish first draft through of enhancer integration section.  On to promoters next. DFISH on cell culture remove … Continue reading →

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Thursday 11/01/12

Posted on November 1, 2012 by admin

9:30 A – 7:30P, 8:30P- 12:00A Software install on STORM2.  Hal can’t detect drivers for national instruments panel. Overview of software install: see post Fly work Collect virgins Sort and cross Espl/sim flies Clear MTDs into 2 new bottles, move … Continue reading →

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