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Tag Archives: genomics
Tuesday 03/12/13
10:00A – 11:15P STORM Finish O/N STORM run on STORM2 Finish O/N STORM run on STORM3 Installing mount and sliders on STORM4 to allow for 3D imaging Lessons: Final appeture (removed for alignment) front-back position chosen to minimize the size … Continue reading
Saturday 11/10/12
10:45 A – 8:00P Snail project stuff Collect virgin Pr/Tm3Z, Sp/CyOZ, and TM2/TM6 (males + virgins) Order primers for screening E(spl) and sim[D]. Also for en and inv introns DNA FISH probe design Wrote new matlab script for concatinating text … Continue reading
Posted in probe and plasmid building, Summaries
Tagged confocal, genomics, planning
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Thursday 10/25/12
9:50 A – 7:00P, 8:30P – 11:20 P Clean and coat coverslips washout fix from O/N fix samples, move to EtOH rehydrate, check for presence of staining. Respond to team about NSF BIRS proposal Pol I arrived 4:00 PM check … Continue reading
Posted in probe and plasmid building, Summaries
Tagged coverslips, genomics, probe making, writing
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Saturday 07/28/12
10:30 A -2:00P, 4:30P-7:30P, 9:50P-11:00P Finish O/N confocal of m197. Imaging plane too high, lower sections of most embryos not fully imaged. Set up rescan. ~10:30A. working on parsing genomic data to test changes in H3K27me3 occupancy in A8 using … Continue reading
Thursday 07/26/12
10:20 A New fly cages of Pc-GFP and yw. Pc-GFP not growing well, should have ~10 bottles/cage. 4 would be sufficient for yw cage. Crossed virgin 3x mat Gal4 x Pc-shRNA2 in vial test viability. new bottle of 3x mat … Continue reading
Posted in Summaries
Tagged DNA-FISH, genomics, in situs, sectioning
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