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Tag Archives: images
Thursday 9/26/13
10:00a – 10:40p Goals Laundry email Ting & Co. draft letter on progress on goals on chromatin Analyze existing data for new spots: density, puncta etc. scale factors? (density vs. length or something?) Computer splitdax aborted in night when ProRAID … Continue reading
Posted in Summaries
Tagged computer, images, Library2, probe making
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Tuesday 09/24/13
11:00a-4:30p, 8:00p-11:00p Goals: Cell culture plate cells on Poly-Lysine coverglass (with Bogdan ?) passage remaining cells (with Bogdan ?) Prep 9/2 plate and new plate for DNA FISH. Staining O/N primary staining of Pc and anti-flag. Probe making RNA cleanup … Continue reading
Monday 09/23/13
9:30a – 7:30p, 9:00p – 11:50p Meetings 10a, lab meeting 11:45a, meeting with Ting and Fred Need to send letter to team. Cell in situs Wash out primary probes STORM E6 (tertiary approach still) — very weak spots visible in … Continue reading
Thursday 09/19/13
9:00a – 7:45p, 10:00p – 11:00p Writing Referee work working remotely (9a-12p) write up comments BJ review Goal: Trim down BJ review by 3 of 16 pages started trimming from beginning. 1 full page shorter. mRNA indexing human genome 22917 … Continue reading
Wednesday 09/18/13
9:15a – 11:30p Writing working on BIRS workshop report (9:15a- 12p) Probe making / training with Bogdan test PCR from yesterday common primer concentration was a bit low gel didn’t fully dissolve, might have affected gel running: Repeat PCRs. Repeat … Continue reading
Posted in probe and plasmid building, Summaries
Tagged coding, images, Library2, probe making
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Wednesday 09/11/13
9:30a – 11:30p Review submit review submit copyright agreement BIRS draft email of final reminders contact Vistas Dining hall about meal costs and reserving tables Print driving directions to and from Airport/Banff, Lakes, and hotel. Print hotel receipt Probe making … Continue reading
Sunday 09/08/13
Goals Run gel to test RT reactions from plate-based T7 (complete) second proof read of review article, prepare to submit (not complete) STORM imaging (complete) Review started rereading and editing replies to reviewers. Results E6, E7, E10, F6 with P1 … Continue reading
Wednesday 09/04/13
8:15a – 4:50p, 9:30p – 12:00a Discussions discuss project 2 troubleshooting with Hao and Jeff discuss with Jeff filtering STORM data on molecule properties (such as width photon number correlations or average intensity per flash as a function of space). … Continue reading
Protected: project 2: update 09/04/13
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Library analysis
Labeled version of gel: thoughts small sub-libraries preferentially lost? is this because we only get a fraction of the probes in each sublibrary to amplify? more likely a relative abundance issue — should have substantially more mass in large libraries. … Continue reading