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Tag Archives: mRNA counting
Protected: collating snail counts
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Protected: Feedback/followup from lab meeting
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Posted in Fly Work, Research Planning
Tagged Fly crosses, mRNA counting, sna, to do
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Protected: Gal4 gradient LacZ response
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Posted in Transcription Modeling
Tagged activators, Gal4, mRNA counting
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Friday 07/01/11
10:00 A – 6:00P Analyze sna data MP06 Hz at cc13-14 transition, substantially less y. Seems to be a temporal transition. Analyze G4B LacZ data (see p0st)
Thursday 06/30/11
9:30 A – 4:10 P Data analysis: focus on sna rescues. Imaging: sna 2.8 enhancer rescues. Partially cooled lasers/lens lead to drift during image acquisition. Stepsize is substantially greater than 330 nm and mRNA are not detected in consecutive layers … Continue reading
Protected: Issues with detection sensitivity
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Posted in Microscopy, Shadow Enhancers
Tagged controls, mRNA counting, sna, y-BAC
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Protected: sna rescue, shadow/distal removed, dramatically lowers expression
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Posted in Shadow Enhancers
Tagged analysis, mRNA counting, rescue, sna
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Protected: Weaker effect on reporter when removing distal sna enhancer
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Posted in Shadow Enhancers
Tagged analysis, mRNA counting, sna
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Protected: High mRNA expression upon proximal sna enhancer deletion
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Posted in Shadow Enhancers
Tagged analysis, mRNA counting, sna
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Tuesday 06/28/11
9:30 A-7:00P, 7:40-8:40, 10:00P- 11:10P Data alignment Flip sna[D] and hb[D] flies into new bottles, keep at 25C. Compare hb data by image type. confirm fairly dynamic expression prevents easy super-position of patterns during cc13. February’s data format doesn’t fit … Continue reading