Tag Archives: probe making

Monday 12/16/13

Posted on December 16, 2013 by admin

9:50a – 1:00a Group meeting 10a – 2p see notes Probe making cy7 dye from Lumipore arrived A750 labeled IDT primers arrived first test gel — try old gel of Bogdan’s (11/18). Ran smeary and fast, primers ran off the … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Monday 12/16/13

Sunday 12/15/13

Posted on December 15, 2013 by admin

11:00a – 9:00p z-calibration some new ideas for finalizing new z-calibration routine: see if different starting z-position affects curve alignments (isn’t that the whole hypothesis in the first place?) well, all the data comes from the same range, but the … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Sunday 12/15/13

Saturday 12/14/13

Posted on December 14, 2013 by admin

11:15a – 2:30p, 6:00p – 11:45p Coding matlab-storm test installation: created new ZhuangLab account Install for Guipeng. DaoSTORM objects to spaces in pathname. adding double quotes ” does not fix. library Prep New function (in Genomics Functions toolbox) function Seq … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Saturday 12/14/13

Friday 12/06/13

Posted on December 6, 2013 by admin

9:50a – 10:45p Goals Presentation prep for meeting with XZ (meeting rescheduled) Coding: work on new z-calibration algorithm add run silent options to STORMrender update template ini and xml parameters to contain new ROI defaults Probe Making HPLC cy7 secondary … Continue reading →

Posted in Summaries | Tagged | Comments Off on Friday 12/06/13

HPLC 12/06/13

Posted on December 6, 2013 by admin

DNA labeling 60 nmol of DNA in 100 uL .1M NaHCO3 (100 mg in 12 mL) .1 – .2 mg dye in 50 uL DMSO (50 uL of 1-2 mg / mL) React at 37C for 1-2 hrs (shake occassionally). … Continue reading →

Posted in probe and plasmid building | Tagged | Comments Off on HPLC 12/06/13

Wednesday 12/04/13

Posted on December 4, 2013 by admin

9:40a – 11:30p Goals show Hao new project 2 results RNase-Away / bleach treat everything on my bench fix and stain new RNA cells. Allow 1 hour prehybe. Project 2 See notes Chromatin colors, double stain prep Probe organization reorganized … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Wednesday 12/04/13

Wednesday 10/23/13

Posted on October 23, 2013 by admin

9:15a – Deep seq prep / discussion Miseq = 25M reads. Have almost 92K unique sequences in my library, so need 1M sequences just for 10 fold coverage. recommend run all libraries at equal concentration — we’ll get a lot … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Wednesday 10/23/13

Tuesday 10/22/13

Posted on October 22, 2013 by admin

8:30a – 10:30p Goals Ph simulations: time and optimize? Ph simulations: add Ph-Flag species Analyze qPCR data chromatic warp computations for Bogdan Banff report RNA prep/ clean up RT reaction cast gels with Bogdan test RT reaction qPCR analysis for … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Tuesday 10/22/13

Friday 10/11/13

Posted on October 11, 2013 by admin

10:00a – 10:30p Goals send Ph modeling results to Ajaz work on presentation send reminder about racing Saturday STORM analysis Copying F11 data to ProBox2. Delete data from Storm2 Running splitdax on no405Primary, E07fail, F09, and F01v2 (latter 2 including … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Friday 10/11/13

Wednesday 10/09/13

Posted on October 10, 2013 by admin

9:00a – 12:35a Goals work on Presentation for Stowers STORM Ran out of disk space, crashed O/N Run lost focus anyway immediately after position 0. Probably not enough oil. Possibly positions were chosen too far apart. Need to explain these … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 10/09/13