Tag Archives: sectioning

Friday 07/06/12

Posted on July 6, 2012 by admin

10:10 A – 7:30P, 9:00P -11:15P Flip fly cages, 10:10A Revisions of dynamics paper, revised, updated to v7. compare to NM requirements.  Version and comparison sent back to ML.  May need to combine figures and prune text. Take embryos out … Continue reading →

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Thursday 07/5/12

Posted on July 5, 2012 by admin

9:50A – 7:15P, 9:00P – 10:45P check nascent transcripts of AbdA (red) and Antp (green) on yw and Pc O/N short-fix embryos.  No obvious staining on turnkey. Should check 6/11 embryos as positive controls and 6/25 embryos to see effect … Continue reading →

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Wednesday 05/30/12

Posted on May 30, 2012 by admin

12:00 P -8:00P clean and coat 2 racks of small coverslips confocal analysis of embedded oligo-paint sample.  Embryos not detectable. sectioning of Su(Z)12-750 K27me3-647 + Dm0-750,647 working on new vector version STORM analysis code.

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Tuesday 05/01/12

Posted on May 1, 2012 by admin

11:00 A – 6:45P, 8:00P -12:00A flip fly collection cages, 11A Miniprep cultured colonies: 8E, 7E, 2E, AbdB2 and Fab8 (2 colonies each).  Send for sequencing. Rearrange ultratome room, suspend on air-table, clean room. Flip collection cages, 3:15 A Analyzing … Continue reading →

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Thursday 05/26/12

Posted on April 26, 2012 by admin

10:00 A – 11:45 P Check colonies from Bx-C round 1 cloning: all plates look good, no lawns. Gel-extract linearized Taf, Tub Gel-extract newly cloned Bx-C probes Antibody synthesis: antibodies 61-69 Flip collection cages: 10:15 A Washing out primary antibody … Continue reading →

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Monday 04/23/12

Posted on April 23, 2012 by admin

9:45 A – 11:30P Lab meeting presentation John Wu’s thesis defense Consolidated disk drives: new data on external drives Alistair4 and Alistair5 backed up to empty gaps slots on Alistair2 and 3. Flip fly cages – 2:30P Analyze Psc localization, … Continue reading →

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Sunday 04/15/12

Posted on April 15, 2012 by admin

4:30 P – 11:00P STORM imaging. HP1-750 slide 1 not fully etched, only some nuclei switch. 1hr 15 min etching. STORM imaging Hp1-750 slide 2 over-etched, 750 switching great, 647 dye almost all lost, very weak by conventional and few … Continue reading →

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Wednesday 04/11/12

Posted on April 11, 2012 by admin

10:00A – 11:30P coating 5o mm coverslips put coverslips in metal, vertical mount holder.  Don’t stay balanced in ceramic holder.  Need to alternate positions or the coverslips stick. Do coating in 400 mL in 1000 mL beaker (not 300 mL … Continue reading →

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Thursday 04/05/12

Posted on April 5, 2012 by admin

10:00 A — 8:30 P Take GFP embryos out of 4C, incubating at RT heating up 2x SSCT for hot wash mounted oligo-paint embryos before and after washes (with WGA-488 for post-wash embryos).  Remaining embryos at 4C Cut sections of … Continue reading →

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Tuesday 04/03/12

Posted on April 3, 2012 by admin

10:00 A – 11:00P washing out primary antibodies (after 10 hrs at 4C) maybe should do longer, return to 1 hr RT followed by O/N 4C? We’ll see how these ones look. Confocal images of embedded embryos for sectioning today … Continue reading →

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