Tag Archives: sectioning

Friday 03/16/12

Posted on March 16, 2012 by admin

9:00 A — 9:30P Finish O/N confocal run, save data Projecting data in Matlab Make new and autoclave: 2x YT aliquot IPTG and X-Gal Transform and plate competent cells, 5:30 plates at 37C O/N.  300 uL per plate PCR with … Continue reading →

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Friday 03/02/12

Posted on March 2, 2012 by admin

10:05 A -1:00 A Flip new PcG shRNA lines. Enter into stocks. Screen MP12 rescue crosses.  set up 6 new crosses, 3 with bright eyes might be homozygous MP12. 1 vial with straight wings tossed and 1 vial with Sp … Continue reading →

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Thursday 02/23/12

Posted on February 23, 2012 by admin

10:05 A – 12:10A PcG and Bcd staining embryos incubating in primary at RT for final hour. Flip fly plates Discussion with John about projects Need to look up Drosophila repeat sequences for probe testing. in situs day 1 on … Continue reading →

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Wednesday 02/22/12

Posted on February 22, 2012 by admin

9:30 A – 8:50 PM Sectioning: 300 nm. trapazoids work better, long edge at bottom. Cut 3 coverslips of S6.  Two at 330 nm, one at 100 nm. Coverslip 1 at 330 nm is tiled from upper left to lower … Continue reading →

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Saturday 02/19/2012

Posted on February 20, 2012 by admin

10:00A – 5:00P washing out final antibody from in situs. embryos moved to ethanol WGA staining Hox in situs 1:10 set up to try cutting thicker samples.  Using Yari’s new walled area for microtome. select Images for Cell feature on … Continue reading →

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Friday 02/03/12

Posted on February 3, 2012 by admin

10:10 A – 9:30 P Flip collection cages, 10:15 A Prep new coverslips (2 racks) Flip collection cages 12:15 P clear sna[D] flies for virgin collection Flip fly stocks Flip MP09 and 4x snail flies transfer laid embryos to make … Continue reading →

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Thursday 02/02/12

Posted on February 2, 2012 by admin

9:30 A – 7:00P, 8:45 – 12:30A List of contacts to write to for BIRS, sent to co-organizers Flip fly cages 10:15 A.  Keep plates. Turn on humidifiers for sectioning room, 10:30A Check baked resin in chamber slides on confocal. … Continue reading →

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Tuesday 01/31/12

Posted on January 31, 2012 by admin

10:00 A — 12:55 A Flip fly cages (MP08 and yw) Review JCC app. Submit JCC app. Working on snail paper final results/model section. Syncing datafiles on snail patterning sectioning: Cut 2 slides of s04(13-introns), one of slide 1, one of … Continue reading →

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Friday 01/13/12

Posted on January 13, 2012 by admin

10:10 A -8:00P Start etching K4 Flip MP08 cage (10:40A) New MP08 cage cut eng-dig sections 3 – 4:30 cut new Ftz, eng labeled embryos 4:30 -6p, section’s of previously trimmed block do not hold together well. cut selection of … Continue reading →

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Wednesday 12-21-11

Posted on December 21, 2011 by admin

7:40 A – 11:00P breakdown O/N confocal of MP08 write script to extract nuclei from cytoplasmic localized mRNAs (sog and snail).  Extract nuclei for s05_MP08_b. first pass dot counting for s05_MP08_b and for snaD_b.  Data not analyzed yet. cutting samples … Continue reading →

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