Tag Archives: STORM analysis

Friday 09/06/13

Posted on September 6, 2013 by admin

8:50a – 5:10p, 8:30p – 10:40p Other stuff write to landlady about move in return keys for 2nd floor Coding fixed bug in STORMfinderBeta.m For Hao for updates: Fork fast-forward https://github.com/blog/266-fast-forward-your-fork Data Analysis copying files to ProRAID while running splitQVdax … Continue reading →

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Sunday 08/25/13

Posted on August 25, 2013 by admin

2:45p – 7:15p Review revising introduction of models Cell culture clone8 cells growing fast. A bit overgrown, need to split this afternoon. separated ‘non-adherent’ fraction of Kc167 cells remain primarily non-adherent. Adherent fraction of remains adherent. test plate Kc167 cells … Continue reading →

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Saturday 08/24/13

Posted on August 24, 2013 by admin

4:00p – 6:30p (420 regatta day) Coding Turned splitQVdaxFast into a function. Overwrote splitQVdax original, made that file into splitQVdaxM (for mem-map). overall it looks like memory mapping dax files is a bad idea. They are too big. Running new … Continue reading →

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Thursday 08/22/13

Posted on August 22, 2013 by admin

10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading →

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Sunday 08/18/13

Posted on August 18, 2013 by admin

12:20p – 7:00p, 8:40p – 11:30p Goals reread and edit review analyze latest round of BXC data Probe making verify direction of MYcroArray BX-C Hox probes: probe region is “+” strand (antisense) T7 is on the nick side primer –> … Continue reading →

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Monday 07/22/13

Posted on July 22, 2013 by admin

9:30 A – 7:00 P, 8:30 P – 11:50 P STORM Finish O/N set up new STORM, coverslip 7. high background, must by cy5-primary. not too much switching, maybe single labeled cy5 primary. Responsive to 405 but only at substantial … Continue reading →

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Thursday 07/11/13

Posted on July 11, 2013 by admin

10:00 A – 7:00P, 8:30P-10:00P STORM Finish O/N run of PhM-flag-488, Ph-647 take bead images transfer data to ProRAID Set up analysis to run on Cajal Embryo in situs wash out probes check staining: All embryos washed off! 1 tiny … Continue reading →

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Friday 05/31/13

Posted on June 1, 2013 by admin

10:00 A – 7:00P, 9:30P – 12:30 A Check RNA hybes on STORM2 (hybed at 50C (55C?) in dry oven O/N in coverglass preps like for DNA labeling) (quick check on turnkey looked promising) tub looks good but hard to … Continue reading →

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Wednesday 05/08/13

Posted on May 8, 2013 by admin

9:30 A — Probe making pool digests aliquot out test DNA: 1 uL digest DNA, 3 uL TBE, 1 uL 6x loading dye + 12x gel green, 5 uL formamide with 5% 5M EDTA Pour new 16% urea PAGE gels … Continue reading →

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Friday 04/05/13

Posted on April 5, 2013 by admin

9:00 A – 8:00 P, 9:00 P – 10:45 P General (8:00 A allergy test) STORM analysis Getting images of relative background levels in 3-Cy5 vs 405-cy5 Drift correction makes sparse images look horrible (even the rather long integration of … Continue reading →

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