CONTI grant evaluation
- first: Tako Hensch, microRNA — AGO2 is imprinted
- Lichtman then Dulac then us?
Bogdan Talk: Chromatin structure
- ‘identical’ probably too strong for imprinted genes
- one allele is 1.5-2x larger than the other in imprinted tissue
- look at this ratio for non-imprinted genes (or same genes in non-imprinted tissue).
- whose maternal whose paternal?
- imprinting is highly tissue specific
- too long (maybe 30 min?)
- intro is good (CD). why is structure important at length scale of genes (interactions matter?)
- cut the internal organization scaling laws.
- quantification of multiple domains for interaction (within and between domains).
- intermixing between domains needs a contrast.
- XZ likes the schema of why dense chromatin is harder to transcribe.
- conformation changes, cell-specific differences (single cell vs bulk).
- kidney vs. brain validation (not)
- show well studied example for which there is data (bulk).
- large scale, single cell, 3D study. — imprinting in brain is 40:60 – is this per cell or only in average?
- IGF H19 data
- preliminary but intriguing difference between allele size.
- mark what’s labeled (full cluster of H2 IGF)
- link spidery domain to active (just say provocative similarity)
- don’t mention loops
- feedback to follow
- use Allen Brain atlas to motivate one gene at a time. No correlation analysis
- emphasize single cell resolution, high detection efficiency.
- much too long.
- less technical validation, more emphasis of the scale.
- behaviors in general
- instinctive behaviors — genetically programmed?
- Genes known for behavior
- The Medial Preoptic area of the hyothalamus: Distinct neurons active in the MPOA in different behaviors (Male infanticide, male parenting, male agreesion, male mating, female mating, female parenting)
- many definitions of cell types: propose that many of these are governed by combinatorial code of genes.
- RNAsin added to tissue to co-stain with antibodies.
- differences in stains not so evident.
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