Hb (green) and Bcd (red) double stained (early cc13?)
zoom , showing subnuclear localization patterns.
showing very gradual hb transition
hb (after applying mask), used in calc above. mask is much easier to make using Cm-diI inverse than using a nuclear dye.
Bcd (after applying mask)
nuclear dye labeled cycle 14 embryo (note nuclear density).
bcd red (blue trace below), hb green.
note much sharper transition in cycle 14 embryos