10:00A – 6:30P, 7:50P – 11:30P
- Order flies from Bloomington, sim deletion: 7646, Espl rv1: 199
- # start expanding sna[18] and double balancer on 3rd.
- request Esc mutant flies from Vivek
- wrote to Markstein, try to get in touch with TRiP about no phenotypes of PcG knockdowns using TRiP lines.
- Etch 20 min + 10 min Pc-647, GFP-750 slide.
- Work out crosses for E(spl), sim double mutants (see post)
- Realign quadview (drift, started to clip image).
- STORM imaging of Pc-GFP
- rewrote CoV analysis to fit sigmoid of a gaussian to the pattern first and use this to determine in and out of mesoderm.
- see figs