crosses for E(spl) sim double mutants


Large cross of: sim[D]/Tm6b,Tb  x Espl[D]/Tm6b,Tb

take non-Tb progeny and cross to double balanced 3rd (e.g. Tm3/Tm6)

Cross individual F2s to double-balancer on 3rd.  Expand these single fly lines (1 generation should be enough).  PCR across the deletions to screen for double mutants.

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