Friday 10/12/12

9:45 A – 7:00 P, 8:00P-9:15P

Fly work

  • collect virgins: E(spl)[D], sim[D], MTD
  • new bottle cross: MTD virgins x male TRiP Scm shRNA
  • 5 new vials cross E(spl)[D] x sim[D]
  • collect PM virgins E(spl) and sim
  • MTD moved back to stocks.

Data analysis

  • Annotating valid nuclei from automatic recognition (with confidence estimates) (10:30 – 12:30)
  • summary of dynamics

DNA FISH: Day1:

  • reuse oligo-3R probe (30 uL, diluted into 30 uL of embryos
  • 100 uL (1:10 dilution) of cy5-centromere dye in 100 uL of embryos
  • 15 min denature at 80C, 10 min probe denature at 95C, mix
  • and incubate at 37C O/N. ┬áIn 1.5mL eppendorffs in ependorff heater/shaker.
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