10:30 A – 1:45P, 4:45 – 8:15P
- 10:30A collect virgin Espl/sim
- 12:00 P, flip collection cages
- 7:30 P, fix embryos, 0.5 – 7 hrs MTDs, Psc 1, and Pc 3. Left on shaker. Good embryos should be sunk in MeOH layer and preserved.
- forgot evening virgin collection.
- Finish O/N confocal. 2 postions definetely not sampled deep enough. A few past nuclear layer but not past snail. Definitely dynamical structure to the apical localization of snail mRNA.
- Finish staining Abd-B (405,750 and Cy3B) and WGA-488 and Draq5. MTD x Psc 1 and MTD x Pc 3.
- Wash out probe at 60C hot wash (2x SSCT) and RT SSCT, followed by PBT washes.
- labeled with WGA-488. At 4C in PBT, check staining tomorrow.