First small test probe
- The primer has a 5′ TYE665 dye.
- Both primers are at 200 µM (200 pmoles/µl).
- template was at 10 ng/µl.
A look at the genomic region
Question for Ting /Brian
Randomly ligate labeled primers with DNA probe fragments. Way to use modified terminal nucleotides so that primers don’t ligate together. HPLC to separate out the labeled labeled single strands.