10:00A – 1:00P, 6:00P – 7:45P, 9:00P – 11:00P
Embryo Section staining
Control test with AATAT worked.
- No 92C pre-denature
- 60C 25 min
- minimal cooling gap. Add probe to slide, drop coverglass on, seal
- 94C heat-block 2.5 min denature
- 37C O/N incubation
- cold 2x SSCT wash (45 min).
- label with DAPI, check. works!
Try post labeling with AbdB
- 1 hr block (using Colenso’s Donkey serum at 1:5 dilution. should confirm this)
- 1.2 hrs primary
- quick block
- 2 hrs secondary
- PBT rinse in coplin jar
- check staining: DAPI / cy5 channels intact, no staining at all in Cy3 and no background. (might have been the serum?)
Try post labeling with RNA in situ probes:
- 50:50 PBT hybe solution in incubation chamber at RT
- 20 min at 42C incubation chamber in hybe oven.
- en-bio, inv-bio, AbdA-dig, Abd-B-dig.
- chromebook remote desktop powerpoint / impress display: Changing Monet primary display to 1360 x 768 at view full screen on chrome when gives best screen filling presentation when centered (moving mouse to edge of screen will still browse out a larger space, presumably because of monitor 2. Unplugging monitor 2 also helps.
- hold down windows + tab + p brings up a screen selector. Shame chromebook has no ‘windows’ key.
- Can indeed select “show desktop only on 1” This works best.
Coding To do
- Can we make STORM-render resizable?
- will this work with adding buttons? — could add a get position command for the parent box.
- Just need to set units to relative after the object has been placed using the chosen units system (e.g. characters).