9:30a – 1:00a
- Stain new cells: ANT-C, G05, D12
- chromatin probe synthesis debugging
- order new Maxima and T7
- create list of probes to make next
- start compressing bead data! – postponed again.
- Finish O/N STORM of F04-A647, F03-A647
- start analysis of F03,F04 and F04,F03 data
- Finish running ChromatinCropper on F10 data
- update and reboot Monet
- Working on slides for next meeting with XZ: full Lib2 data update
Coding Genome analysis
- adding gene viewer:
- now built into the
- Note current version of flip gene only flipped complete genes, not genes that edged into the region.
- Fixed in
- constructed maps for all of Lib2
- Multi-color display works fine.
- single color loading of demo data fails.
- fixed single color loading — imagesc won’t write indexed images (i.e. 2D matrices, not RGB data) to axes in a GUI (I just get a solid colored field. I can change the color, but not actually get the image). Sending the same command to the GUI
handles.axes2; images(I);and to
figure(1); imagesc(I)back to back in the same code produces the image correctly in the figure and as a solid field in the axes. As soon as the image is an RGB image it displays correctly in both. WEIRD matlab. weird.
- (simple work-around, use
ind2rgb(I,map)to convert first. Only it’s pick about the range of the map, the data type of the map (needs a
double, even though you can call a color map on a
uint16, it gives weird results). Also
double, which doesn’t seem obviously scaled, so I use
makeuintto convert these to balanced contrast
- at some point I want to get rid of the automatic balanced contrast, or make it a toggleable function rather than an intrinsic property.
- ordered new Maxima (full length)
- ordered new T7 kit
- ordered new ProRAID (4 bay) — easier to fit on desk, no adventage with 8 bay sincy they max out the RAID 5 array at 4.