Journal club: whole animal light-field microscopy

(Prevedel et al Nature Methods 2014)

Light-field microscopy –

  • capture 3D strcuture of object in single snapshot
  • first developed in 2006
  • array of micro-lenses, different parts of sensor get different z-fields
    • going deep spread out image, lower signal to noise, sacrifice SNR /x-y resolution for time resolution

Light field deconvolution microscopy

  • goal: use deconvolution to improve the x-y resolution that gets compormised by the low SNR
  • put beads into agarose, measure resolution on scale of 1 um (little worse) (?)
  • calcium imaging (chemical indicators or genetically encoded indicators (Preicma, GCaMP).
    • Ca dyes best for change in Ca conc, not so good to measure aboslute levels.
    • this paper uses GCaMP.
  • image whole worm, 30 um deep.
    • don’t actually separate neighboring cells. But do get individual cells resolved over substantial depth.
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