(Prevedel et al Nature Methods 2014)
Light-field microscopy –
- capture 3D strcuture of object in single snapshot
- first developed in 2006
- array of micro-lenses, different parts of sensor get different z-fields
- going deep spread out image, lower signal to noise, sacrifice SNR /x-y resolution for time resolution
Light field deconvolution microscopy
- goal: use deconvolution to improve the x-y resolution that gets compormised by the low SNR
- put beads into agarose, measure resolution on scale of 1 um (little worse) (?)
- calcium imaging (chemical indicators or genetically encoded indicators (Preicma, GCaMP).
- Ca dyes best for change in Ca conc, not so good to measure aboslute levels.
- this paper uses GCaMP.
- image whole worm, 30 um deep.
- don’t actually separate neighboring cells. But do get individual cells resolved over substantial depth.