Category Archives: Fly Work

Fly work

Posted on January 13, 2011 by admin

2x mat-gal4-alpha x UAS-da[M2], twi2, and Espl/m8 Flip collection plates: 1:48 Remove collection plates for aging 4:48 Fix embryos: 5:48 (1-4 hr collection). Collect w; Sp/CyO; Hb[D]/GFP Collect DBTZ and DBTG males and virgins Cross MP04 x DBTZ (2 vials each … Continue reading →

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Fly work

Posted on January 12, 2011 by admin

Flip collection cages: 10:40A Collect virgin DBTZ, DBTG (none), and w;Sp/CyO;Hb[D]/GFP 10:30A Collect virgins and males DBTZ and DBTG, 11:30 P

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Fly Work

Posted on January 11, 2011 by admin

Flip collection cages Collect MP3/CyO; Pr/HbZ and MP11/CyO; Pr/HbZ males Clear DBTG and DBTZ for virgin collection (to cross to MP04s) New crosses: 3A.1/CyO; Pr/HbZ x w; Sp/CyO; Hb[D]/GFP 3A.4/CyO; Pr/HbZ x w; Sp/CyO; Hb[D]/GFP 11A1.1/CyO; Pr/HbZ x w; Sp/CyO; … Continue reading →

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Fly work

Posted on January 10, 2011 by admin

Found 2 more fog rescues males.  (for 3 out of 109).  (Expect 1:2). Collect UAS-twi-twi males and cross to 2x Gal4-ma virgins. Collect UAS-da males and cross to 2x Gal4-ma virgins. Collect UAS-Espl males and cross to 2x Gal4-ma virgins … Continue reading →

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Fly work

Posted on January 7, 2011 by admin

Collect virgin w;Sp/CyO;Hb[D]/GFP fog- 2x  MG-alphas Dostatni will send: stock that has 4 insertions of the pnanos-Gal43GCN4-Bcd3’UTR transgene (1 on each 2nd chromosome and 1 on each 3rd chromosome). Lab fedex account is closed.   Kevin will set up an … Continue reading →

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Fly work

Posted on January 6, 2011 by admin

Collect virgins, w;Sp/CyO;Hb[D]/GFP and 2x MG-alphas (9:30 A) Collect virgins, w;Sp/CyO;Hb[D]/GFP and 2x MG-alphas (9:00 P) Flip fog into new bottles to expand.  Start collecting virgins tomorrow. w;Sp/CyO;Hb[D]/GFP have mites.  Need proper lids (probably acquired during break when old food not … Continue reading →

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Fly Work

Posted on January 6, 2011 by admin

Flip all fog vials.  Start collecting new fog virgins (probably won’t have enough emerging flies to find rescue males in the previous round.   If I do, I’ll need fog virgins to try to create a stable rescue line). Collect … Continue reading →

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Fly Work

Posted on January 5, 2011 by admin

hb[12]/GFP x DBTZ males and virgins Perry collected, need to sort and cross (in order to make hb[12]/TM3-LacZ line: crossed 4 vials, including 1 cross of orange eyed (just the DBTZ hsp w+?). some 3/CyO; Pr/HbZ actually look okay. 11/CyO; … Continue reading →

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Fly Work

Posted on December 19, 2010 by admin

Move non-expanding stocks to 18C for break. Move 25C stocks to 22C for break. Flip MP11; HbZ and MP3; HbZ backup vials.  Flip ac, and ac,sc bottles.  Freeze fly collection cages.

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Fly Work

Posted on December 17, 2010 by admin

Give Perry vnd[D38] and brk[M68] gene deletion stocks. Flip collection cages for MP11/CyO, MP3/CyO, sna[18]/CyO; MP10s, 2:30 P Cross hkb::fog stock 1 x fog null virgins (10 vials) at 22C Organize stocks for hb rescue to set up crosses over … Continue reading →

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