Tag Archives: fly work

Monday 04/07/14

Posted on April 7, 2014 by admin

9:30a – 11:30p Meetings lab meeting (see protected notes) my journal club presentation (see google-docs presentation) initial planning meeting with Steven — need joint write-up, goal to write by Wed Writing finalizing write-up 1 for XZ with Jeff. working on … Continue reading →

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Saturday 02/08/14

Posted on February 8, 2014 by admin

10:30a – 4:45p, 10:00p-11:50p Chromatin Deep sequencing of libraries finish gel extraction aborted last night due to mini-centrifuge failure. Data analysis Analyze new ANTP data issue: need to fix integrated drift correction, have some weird errors interpolation was not correct … Continue reading →

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Sunday 01/12/14

Posted on January 12, 2014 by admin

4:00p – 5:20p, 5:50p – 10:30p chromatin data analysis F03, F04, F05, and BX-C data does current spit dax work correctly on 512×256 images? Or are half the frames skipped? or something worse? seems to work just fine. Ran bead … Continue reading →

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Wednesday 09/25/13

Posted on September 25, 2013 by admin

9:30a – 11:15p STORM stop O/N STORM start copying data to Monet from O/N start spot-finding analysis on yesterday’s O/N splitdax F6 data. G2 data also not analyzed: set up DaoSTORM analysis on Cajal G2 bead data looks sparse — … Continue reading →

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Friday 03/22/13

Posted on March 22, 2013 by admin

11:00 A – 7:30 P, 9:30P – 11:58 P Discussion recommend using homology-fused single labeled probes to test the double labeling efficiency for Hao’s experiment. reference call for Bryon Genomics cleaned up Genomics functions maxInterval.m maxIntervalBed.m added new function IntervalFuseGaps.m … Continue reading →

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Failures update

Posted on March 1, 2013 by admin

Fly Genetics Failed knockdown: 2x Mat alpha tub driving any of the TRiP valium 20 or valium 22 PcG target shRNAs. Gives viable F1 and F2s or infertile F2s. No PcG phenotypes. Kill all current crosses Kill all expanding Gal4 … Continue reading →

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Tuesday 03/26/13

Posted on February 26, 2013 by admin

10:00 A – 6:30P, 7:50P – 10:20P Embryo work Flip collection cages ~10:00 A No hatching from Mat apha tub x Rad21, Scm, Pc(3), Psc(1) shRNAs. all these embryos appear unfertilized. Frequently moderate deformaties to egg shape, substantial variation in … Continue reading →

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Tuesday 02/19/13

Posted on February 19, 2013 by admin

10:00A – 1:40P, 3:30P-12:10A Computer restart required for hotswap and to switch Alistair8 back to eSATA delete HB data from Alistair3b (now on Alistair3). disconnect Alistair3 future disk shuffling of earlier data might be better done on Tuck? remove Buffalo … Continue reading →

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Sunday 02/17/13

Posted on February 17, 2013 by admin

STORM Finish O/N run of BXC + flank try2 (3D this time). Did not lose TIRF. Did not overfill drive or crash computer (4GB left on hard-drive, 400 written). Copying to new Alistair10. (will move to the new Alistair9 when … Continue reading →

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Saturday 02/16/13

Posted on February 16, 2013 by admin

10:30 A – 8:30P, 10:00P – 11:55P STORM Analysis DaoSTORM analysis still running on Fab7-A647 Pc-750 Read write steps from USB connected hard-drive very slow when this analysis is running. (I’m not sure how much slower the analysis goes to … Continue reading →

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