Tag Archives: staining

Saturday 03/24/12

Posted on March 24, 2012 by admin

10:15 A Assemble draft protocol of embryo multiplex RNA-Protein STORM send to Stacie at Stowers. 5 mL of culture in probably too much to get good aeration in small round-bottom tubes.  Try 2 mL next time.  use Zhuang shaker, it … Continue reading →

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Wednesday 02/29/12

Posted on February 29, 2012 by admin

9:45 A — 11:00P Washing out O/N 4C antibodies from new protein stains Flip Fly stocks Flip vials and enter new GFP lines into fly stocks. Start expanding Gal4 bcd-3′ UTR (to try crossing into PcG RNAi lines.  will need … Continue reading →

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Thursday 02/23/12

Posted on February 23, 2012 by admin

10:05 A – 12:10A PcG and Bcd staining embryos incubating in primary at RT for final hour. Flip fly plates Discussion with John about projects Need to look up Drosophila repeat sequences for probe testing. in situs day 1 on … Continue reading →

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Monday 02/20/12

Posted on February 20, 2012 by admin

9:45 A — 10:00 P Transfer embryos into 30% resin Group meeting / BPS practice talks Transfer embryos into 50% resin New collection cages: 4x snail, yw, and MP09s. embryos into 70% resin.  3 hrs, then 100% resin. images uploaded … Continue reading →

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Saturday 02/19/2012

Posted on February 20, 2012 by admin

10:00A – 5:00P washing out final antibody from in situs. embryos moved to ethanol WGA staining Hox in situs 1:10 set up to try cutting thicker samples.  Using Yari’s new walled area for microtome. select Images for Cell feature on … Continue reading →

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Friday 02/17/12

Posted on February 19, 2012 by admin

10:10 A — 6P, 8P-12:30P Finish O/N STORM run Analyze STORM data collect virgins working on alignment for STORM data.  Rigid alignment seems better for the moment. staining embryos, in situs day 3. New scheme for PcG and histone doubles: … Continue reading →

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Wednesday 02/15/12

Posted on February 15, 2012 by admin

9:45 A — 11:00P Etching Dm0-1 slides (25-30 min) for STORM.  (Slide 69 in records) Flip fly cage, 10A Washing out primary antibody in PcG stained embryos in situs for PcG stained embryos: hox and en locus (see records) organized … Continue reading →

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Friday 02/09/12

Posted on February 10, 2012 by admin

10:10 A — 11:20 P move samples from 4C, change 100% EtOH STORM: setup 10x objective for finding samples. substantial offset, need to calibrate. Need to set laser powers to zero but have lasers on before starting dave! Ordered Bloomington … Continue reading →

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Thursday 02/09/12

Posted on February 9, 2012 by admin

9:50 A – 1:00A Remove and keep primaries from all staining reactions (except traditional snail labeling of MP08 embryos) Washing embryos, in situs / staining reactions day 3. new stock of 1x PBT clean frozen fly cages Flip fly cage: … Continue reading →

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Wednesday 02/08/12

Posted on February 8, 2012 by admin

10:00 A – 8:45P in situs day 2 (no KE) antibody test try 2: 15 min fix <24 hr methanol treated embryos stained for PcG proteins. much higher concentration of histone primary antibodies test stain (record) Trial run of new … Continue reading →

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