Tag Archives: cell labeling

Wednesday 02/25/14

Posted on February 26, 2014 by admin

10:30a – 11:00p Chromatin Project Analysis Analysis of multi-color data quick look at G1-G2 data from 12-30. Almost complete overlap of regions. Current multi-color / adjacent region datasets: ‘Q:\2014-01-10_F11-7_F12-6’ ‘Q:\2014-01-10_G01-6_G02-7’ ‘Q:\2014-01-09_F05F06’ ‘Q:\2013-12-30_G1G2’ – 750 survived remarkably well. Should check buffer … Continue reading →

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Tuesday 02/11/14

Posted on February 11, 2014 by admin

9:50a – 12:15a Chromatin Project Deep sequencing QPCR run QPCR quantify results from QPCR C:\Users\Alistair\Documents\Research\Projects\Chromatin\Code\Scripts\qPCR_MiSeq2Prep_140212.m MiSeq Prep sample concentrations / calculating dilutions: orig library raw 10 uL scale reaction: 25, 14, 25, nM dilutions 1:10 diluted: 1.98, 2.0, 2.0 these … Continue reading →

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Monday 01/06/13

Posted on January 6, 2014 by admin

10:00a – 11:30p Meetings lab meeting (post-doc interview). See notes post-doc chat fixing up slides for post-doc chats, moving onto google docs so that the presentation is mobile google docs having issues loading images in presentations that used to display … Continue reading →

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Friday 12/20/13

Posted on December 19, 2013 by admin

10:00a – 12:45a STORM imaging need images of 715 beads to align A750 and A647 channels Making new 715 bead slide at 1:20,000. If this looks good we can try a 2-color bead slide as well. trimming the tape so … Continue reading →

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Saturday 12/14/13

Posted on December 14, 2013 by admin

11:15a – 2:30p, 6:00p – 11:45p Coding matlab-storm test installation: created new ZhuangLab account Install for Guipeng. DaoSTORM objects to spaces in pathname. adding double quotes ” does not fix. library Prep New function (in Genomics Functions toolbox) function Seq … Continue reading →

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Thursday 12/12/13

Posted on December 12, 2013 by admin

10:45a – 11:00p Ph Simulations Troubleshooting extra along bonds. Oops, actually just resulted from plotting the wrong attachment matrix for the given position list. Simulations ran at subsaturating Ph — more total sites than available Ph. Hence there is a … Continue reading →

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Wednesday 12/11/13

Posted on December 11, 2013 by admin

11:15a – 11:45p Ph Project Highly variable radius of gyration measurements for case with binding — suggests not equilbirated. Binding strength probably much to high. Up to .90, run again. Also increased density of K27me3 sites from 50% to 80%, … Continue reading →

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Wednesday 12/04/13

Posted on December 4, 2013 by admin

9:40a – 11:30p Goals show Hao new project 2 results RNase-Away / bleach treat everything on my bench fix and stain new RNA cells. Allow 1 hour prehybe. Project 2 See notes Chromatin colors, double stain prep Probe organization reorganized … Continue reading →

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Sunday 12/01/13

Posted on December 1, 2013 by admin

9:30a – 7:45p, 10:00p – 12:15a Genomics Cajal slowing down (2536 of 4385. Though all 4 variants of stringency is 4x 4385) CPU cap semi-respected, code is waiting for free cpu dips to launch new jobs. Target is 75%. Running … Continue reading →

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Protected: Saturday 11/30/13

Posted on November 30, 2013 by admin

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