Tag Archives: Library2

Wednesday 01/29/14

9:10a – 8:00p, 9:50p – 2:30a Ph project revising manuscript draft working on abstract refocusing discussion of PcG clusters. Need to distinguish between the sparse micron-scale PcG bodies other people have studied and the numerous, nano-scale bodies we focus on. … Continue reading

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Tuesday 01/28/14

9:15a – 10:50a, 12:00p – 10:15p Chromatin Project Chromatin Analysis Data Analysis Continued analyzing D12 data from 10-26-13 up through image 26 (still plenty more dots to image). F06 analyzed up to but not including image 0_2. Developing analysis pipeline … Continue reading

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Protected: chromatin analysis in progress

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Sunday 01/26/14

10:20a – 10:00p Literature reading Polycomb Potentiates Meis2 Activation in Midbrain by Mediating Interaction of the Promoter with a Tissue-Specific Enhancer read Cavalli’s summary Ring1 activator and repressor. Article makes it sound to me like Ring1 is an activator. Just … Continue reading

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Chromatin Project, short-term goals

Issues with current data analysis In trying to get of order 100 loci per chromatin domain I’ve take a bunch of partially imaged edge of focus domains or other less well defined images (e.g. near edge of field of view). … Continue reading

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Protected: Library2 Data Summaries

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Monday 01/20/14

9:40a – 1:00a Goals send fig files to Jeff Upgrade memory on Cajal. Image ANT-C complex analyze black K100 (internal black domain) plots of internal vs. external. Lab Meeting see notes. journal club notes Computer stuff Memory installed on Cajal. … Continue reading

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Saturday 01/18/2014

10:30a – 7:20p, matlab-storm STORMrender online Getting Jeff set up with STORMrender debugging: STORMfinder added paths in initialization, allowing matlab-storm to access depreciated versions of code in folders that weren’t supposed to be on filepath Features to add to STORMrender … Continue reading

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Friday 01/17/14

11:50p – 12:45a, 2:00a – 3:20a (remotely) Chromatin Project Data analysis Goal: Sort data by expression levels. Getting FPKM expression values: Downloaded data from NCBI GEO database from the 25 Drosophila Cell lines paper. Downloading gene name conversion tables from … Continue reading

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Thursday 01/16/2014

9:30a – 1:00a Happy Birthday. Goals Stain new cells: ANT-C, G05, D12 chromatin probe synthesis debugging order new Maxima and T7 create list of probes to make next start compressing bead data! – postponed again. Chromatin Project Finish O/N STORM … Continue reading

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