Tag Archives: data analysis

Wednesday 07/23/14

10:00 am – 12:15 am Chromatin Project Sequential staining snapped coverslip tried mounting half on flow-system anyway this leaks — not enough pressure seal in the gaskets (especially relative to pump pressure of manual syringe) try manual hybes of second … Continue reading

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Tuesday 07/22/14

11:25 am — 11:45 pm (biking Minuteman path, 8:15a-11:25a) Goals Ajaz’s sample project 2 data analysis chromatin data analysis Project 2 analysis see protected post Chromatin Project Data analysis fitting E06 and G2toG4 data trying to analyze BX-C data from … Continue reading

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Saturday 06/28/14

10:45 am – 5:30 pm, 9:00 pm – 11:50 pm Small tasks restart data copying after computer freeze last night (GPU computation abort failed) restart sticky polymer simulations STORM imaging imaging sample C10 (blue 33 kb vg locus). Conv images … Continue reading

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Tuesday 04/29/14

9:30a – 10:00p Computer Cajal troubleshooting computer behaving very sluggish today, denying connections restarted Cajal. also removed all the memory DIMMs and reinstalled them. Cajal now recognizes 48 GB of RAM again (instead of just 24 GB). Project 2 moved … Continue reading

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Wednesday 12/12/12

10:30 A – 7:30P, Data analysis Analyzing Hp1a centromere data Labeling density still somewhat low, centromere certainly not ‘coated’ with Hp1a by imaging. Might be an antibody accessability thing.  Should compare with bio-labeled centromere probe.  I remember when I last … Continue reading

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Tuesday 12/04/12

9:40 A – 7:45P, 9:00P – 1:00A Dfd/tub analysis Break down O/N confocal run 3 channels, speed 6, 22 positions, 17.5 finished in 11 hour run.  contact zeiss rep Casey about changing channels between z-stacks rather than every section, should … Continue reading

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Protected: K27me3 density

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Saturday 10/13/12

11:00A – 6:ooP, 9:ooP-11:00P binomial statistics and confidence intervals discussion with JM Collect virgin E(spl) and sim[D] flies, 4 more vials of crosses. Finished virgin collections and cross-setups for present.  All flies now on RT shelf.  Organizing Matlab STORM library … Continue reading

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Friday 10/12/12

9:45 A – 7:00 P, 8:00P-9:15P Fly work collect virgins: E(spl)[D], sim[D], MTD new bottle cross: MTD virgins x male TRiP Scm shRNA 5 new vials cross E(spl)[D] x sim[D] collect PM virgins E(spl) and sim MTD moved back to … Continue reading

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Friday 09/07/12

9:05 A – 8:00 P O/N STORM lost focus attempt to get bead images aborted.  Issues with focus lock delayed start and Colenso needs to get on the machine. O/N confocal still had lots of issues with z-focus (apparently not … Continue reading

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