11:25 am — 11:45 pm
(biking Minuteman path, 8:15a-11:25a)
Goals
- Ajaz’s sample
- project 2 data analysis
- chromatin data analysis
Project 2 analysis
- see protected post
Chromatin Project
Data analysis
- fitting E06 and G2toG4 data
- trying to analyze BX-C data from last run of BX-C F06. Drive communication extremely slow.
- aborted data transfer from STORM2, speeds up disk communication
- these spots look great (as anticipated).
New probes
- L3 E06 to E09 (4)
- L2 F01 to F07 (7)
- Per well
- 25 uL Phusion
- 15 uL ddH2O
- 3 uL 1:50 library
- 2.5 uL EvaGreen
- 2.5 uL Fwd primer
- 2.5 uL Rev primer
- 8x Master Mix Lib2
- 200 uL Phusion
- 120 uL ddH2O
- 24 uL Lib 2
- 20 uL EvaGreen
- 5x Master Mix Lib 3 1:50
- 125 uL Phusion
- 75 uL ddH2O
- 15 uL Lib 2 1:50
- 12.5 uL EvaGreen
- To do: BXC-pieces
- A11 to C08 (fwd primers)
- E11 to G08 (rev primers)
New stains?
- need to fix more cells