Tag Archives: embryo collection

Wednesday 03/06/13

Posted on March 6, 2013 by admin

9:50 A- 6:00P, 9:00P-10:00P (remotely) General receive and freeze Jeff’s CustomArray order configure Windows Backup to backup onto Drive D. Contact co-organizers about setting up Speaker’s List, Talk Titles, Activities Page, Transport Page. Embryo Work Flip Esc collection cage, ~11A … Continue reading →

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Tuesday 03/26/13

Posted on February 26, 2013 by admin

10:00 A – 6:30P, 7:50P – 10:20P Embryo work Flip collection cages ~10:00 A No hatching from Mat apha tub x Rad21, Scm, Pc(3), Psc(1) shRNAs. all these embryos appear unfertilized. Frequently moderate deformaties to egg shape, substantial variation in … Continue reading →

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Sunday 03/24/13

Posted on February 24, 2013 by admin

11:30A – 5:00P, 6:30P – 8:15P Coding Overhauling all file loading for STORMrenderBeta Single Bin files Loaded through open menu (or toolbar) Multiple Bin files, manual select Prompts user to find a chromatic warp file Allows warp dimension to be … Continue reading →

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Saturday 03/23/13

Posted on February 23, 2013 by admin

10:30 A – 6:20P, 9:30P Embryo collection Flip all fly cages dispose of previous fly plates with new plate recycling and freezing system. Fix embryos 9:30 PM (~3-14 hrs, mostly at raised at 29C). STORM working out parameters for TCEP … Continue reading →

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Wednesday 02/20/13

Posted on February 20, 2013 by admin

10:00 A – 7:15P, 8:30P – 11:15P STORM Samples: BXC cy5 (single primary) and BXC cy5 + 750 secondary BXC-cy5 mounted for imaging 12:00P Realigned optics Taking bead calibration images Working out parameters to do z-scan. Can’t go too far … Continue reading →

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Tuesday 02/19/13

Posted on February 19, 2013 by admin

10:00A – 1:40P, 3:30P-12:10A Computer restart required for hotswap and to switch Alistair8 back to eSATA delete HB data from Alistair3b (now on Alistair3). disconnect Alistair3 future disk shuffling of earlier data might be better done on Tuck? remove Buffalo … Continue reading →

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Sunday 02/17/13

Posted on February 17, 2013 by admin

STORM Finish O/N run of BXC + flank try2 (3D this time). Did not lose TIRF. Did not overfill drive or crash computer (4GB left on hard-drive, 400 written). Copying to new Alistair10. (will move to the new Alistair9 when … Continue reading →

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Tuesday 10/30/12

Posted on October 30, 2012 by admin

9:30 A – 7:30P, 8:30P – 11:50 P Flies Flip collection plates. # Save MTDs to plate into new bottles.  All in one, need high density. Collect virgin Espl/sim, cross to TM2/TM6 males. Collect virgin Act5-gal4 Make new AJ plates … Continue reading →

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Saturday 10/27/12

Posted on October 27, 2012 by admin

10:30A – 8:ooP Stop O/N confocal by 11A. Confocal data check: Late positions start too low.  Not sure why — maybe middle position to close to edge changed microscope positions? Fly Work Collect virgin Espl[D]/ sim[D].   Flip fly stocks … Continue reading →

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Sunday 10/21/12

Posted on October 21, 2012 by admin

11:00 A-3:00P, 7:00P – 9:40 PM upgrade linux laptop to 12.10 Review writing reading /re-reading recent literature White/Barolo/Cohen paper He / Sinha model see post Experiments Rinse out probes from DNA FISH (probes saved – green box) Rinse out probes … Continue reading →

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