Tag Archives: troubleshooting

Protected: project 2, 04/16/14

Posted on April 16, 2014 by admin

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Monday 04/14/14

Posted on April 14, 2014 by admin

9:45a – 9:00p Computer issues Attempted to swap memory into Cajal. Cajal won’t boot up with any of the memory restored Tuck to 6x 8GB DIMMS. Cajal has 12x 4GB DIMMS wrote to IT to help get system back up. … Continue reading →

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Saturday 04/12/14

Posted on April 12, 2014 by admin

9:30a – 4:00p Computer Management attempting to give users rights to share their hard-drives on the network without having full Admin capabilities to do anything they want. Tried adding my ‘User’ account to these groups “Performance Monitor Users” — can … Continue reading →

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Friday 04/11/14

Posted on April 11, 2014 by admin

9:30a – 10:00p Goals Need to finish review Need to make slides for symposium at Penn send revised Troubleshooting sudden failure in staining Troubleshooting staining Background of problem: Stained new cells on Saturday, went to image these on Monday — … Continue reading →

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Wednesday 04/09/14

Posted on April 9, 2014 by admin

9:30a – 8:30p Chromatin labeling stains failed again. No stains yet detected with this batch of cells. maybe it’s a cell prep issue? This F07 probe worked fine before. maybe some reagents went bad in the cell prep? Make new … Continue reading →

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git remove binary files

Posted on March 21, 2014 by admin

See current file size. First need to git gc Alistair@MONET ~/Documents/Research/Projects/Chromatin/Code (master) $ git gc Counting objects: 713, done. Delta compression using up to 8 threads. Compressing objects: 100% (570/570), done. Writing objects: 100% (713/713), done. Total 713 (delta 257), … Continue reading →

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Protected: Project 2 controls 10/20/14

Posted on March 20, 2014 by admin

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Protected: Overview of 140310 L4E4 data

Posted on March 17, 2014 by admin

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Protected: Overview of 140308 L4E3 data

Posted on March 16, 2014 by admin

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RT smears troubleshooting

Posted on January 22, 2014 by admin

A quick look at my history of RT success to ID what started going wrong? Observations not much smearing in early samples. Incorporation rate increases with total amount of mRNA added. Using beads to concentrate mRNA increases production. Using pure … Continue reading →

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