Category Archives: Journal Club

Chen, Tillberg and Boyden, Expansion Microscopy presented by Yari approach label sample gelation in polyectrolye network proteolysis tissue / polymer sample expand in water image super absorbant polymers (used in diapers) sodium acrolyate + acrylomide special probes required. Can’t just … Continue reading →

Presented by George Recent Nature photonics paper on scattering. Imaging deep with a scattering medium … : http://www.nature.com/nphoton/journal/vaop/ncurrent/abs/nphoton.2015.24.html Intro: scattering just want photons that hit target and bounce back, not the ones that scatter of tissue. Also don’t want to … Continue reading →

Hazen: Super-Oscillation Papers Rogers et al Nature Material, 2012 replace lens with a diffraction grating pattern. – bright external ring, central spot less than diffraction size (185 nm) resolve two slits separated by 140 nm. super-oscillations — Functions with only … Continue reading →

advanced CARITY Cell paper, Yang et al (with Cai and Gardinaru, Deisserof former post-doc). Recently developed clearing techniques Clear T, Scale, CUBIC, SeeDB, CLARITY, passive CLARITY, advanced CLARITY, 3DISCO, i3DISCO etc this paper, claims first whole organ imaging Differences different … Continue reading →

Multiplex single-molecule interaction profiling of DNA-barcoded proteins Nature 2014, Church lab, Gu et al presented by Pallav Intro current approaches = protein vs. library rather do an everyobdy to everybody screen. One pot analysis not well-based screen Method single-molecular interaction … Continue reading →

Type II Cadherins drive assembly of direction selectivity presented by Colesno Saines lab, Cell paper how do all these different cells wire up? On-off direction selective ganglion cells get inputs from on and off bipolar cells in distinct innerplexiform layers … Continue reading →

(Prevedel et al Nature Methods 2014) Light-field microscopy – capture 3D strcuture of object in single snapshot first developed in 2006 array of micro-lenses, different parts of sensor get different z-fields going deep spread out image, lower signal to noise, … Continue reading →

Science Basic observation chemical stimulation of neuron, see more B-actin mRNA in axon (2x) also see more B-actin mRNA following gentle protease treatment — propose mRNA was masked do transcription controls and trafficking controls block neural stimulation pathway, also don’t … Continue reading →