Category Archives: Journal Club

Protected: Jun-Han lab meeting

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Journal Club: Expansion Microscopy 06/19/15

Chen, Tillberg and Boyden, Expansion Microscopy presented by Yari approach label sample gelation in polyectrolye network proteolysis tissue / polymer sample expand in water image super absorbant polymers (used in diapers) sodium acrolyate + acrylomide special probes required. Can’t just … Continue reading

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Journal Club: imaging through scattering media

Presented by George Recent Nature photonics paper on scattering. Imaging deep with a scattering medium … : http://www.nature.com/nphoton/journal/vaop/ncurrent/abs/nphoton.2015.24.html Intro: scattering just want photons that hit target and bounce back, not the ones that scatter of tissue. Also don’t want to … Continue reading

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Journal Club: 01/26/15

Hazen: Super-Oscillation Papers Rogers et al Nature Material, 2012 replace lens with a diffraction grating pattern. – bright external ring, central spot less than diffraction size (185 nm) resolve two slits separated by 140 nm. super-oscillations — Functions with only … Continue reading

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Journal Club: advanced CLARITY and iDISCO papers

advanced CARITY Cell paper, Yang et al (with Cai and Gardinaru, Deisserof former post-doc). Recently developed clearing techniques Clear T, Scale, CUBIC, SeeDB, CLARITY, passive CLARITY, advanced CLARITY, 3DISCO, i3DISCO etc this paper, claims first whole organ imaging Differences different … Continue reading

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Multiplex single-molecule interaction profiling of DNA-barcoded proteins Nature 2014, Church lab, Gu et al presented by Pallav Intro current approaches = protein vs. library rather do an everyobdy to everybody screen. One pot analysis not well-based screen Method single-molecular interaction … Continue reading

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Journal Club: 10/06/14

Type II Cadherins drive assembly of direction selectivity presented by Colesno Saines lab, Cell paper how do all these different cells wire up? On-off direction selective ganglion cells get inputs from on and off bipolar cells in distinct innerplexiform layers … Continue reading

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Journal Club 08/11/14

Presented by Shu Mapping Brain with Diffuse Optics Optical methods as alternative to MRI or PET (which are not electronic embedded device compatable, e.g. parkinson’s deep brain stimulator or pacemakers or young children). Compare IR specta at 750 and 860, … Continue reading

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Journal club: whole animal light-field microscopy

(Prevedel et al Nature Methods 2014) Light-field microscopy – capture 3D strcuture of object in single snapshot first developed in 2006 array of micro-lenses, different parts of sensor get different z-fields going deep spread out image, lower signal to noise, … Continue reading

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Journal Club: B-Actin mRNA in neurons (Singer)

Science Basic observation chemical stimulation of neuron, see more B-actin mRNA in axon (2x) also see more B-actin mRNA following gentle protease treatment — propose mRNA was masked do transcription controls and trafficking controls block neural stimulation pathway, also don’t … Continue reading

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