Tag Archives: analysis

Thursday 07/10/14

10:00 am – 7:30 pm, 11:20 pm – 12:10 am Post-doc candidate visit see notes Chromatin project figure making internal domains need examples for figures Multi-color region analysis Black:Yellow F01 :: F02 F07 :: F06 F11 :: F12 G08 :: … Continue reading

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Monday 05/05/14

9:00a – 5:00p Lab Meeting Guest speaker, see post. Multicolor STORM more trouble-shooting observed that 647 images look particularly weak, especially given the brightness of these spots. 15 min of max 405 completely bleaches the 647 spot. (~50K frames). 561 … Continue reading

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Sunday 12/09/12

10:20 A – 7:30P, 9:30-11:00P STORM 750 1600 mA = 18 mW 1.45 mW 405 200 mW set 647 Made new Glox 750 not switching well.  Not sure if it’s just low labeling (405 looked okay but not strong). 647 … Continue reading

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Friday 07/08/2011

8:30 A – 6:00 P ON confocal failed, too much drift. Organizing hb data into lab meeting part 2. writing embryo matching script using wildtype profiles for hb Rewrote anlz_hb_gradient_data.m and Plot_hb_data.m to rely just on raw density rather counts … Continue reading

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Thursday 06/30/11

9:30 A – 4:10 P Data analysis: focus on sna rescues. Imaging: sna 2.8 enhancer rescues. Partially cooled lasers/lens lead to drift during image acquisition.  Stepsize is substantially greater than 330 nm and mRNA are not detected in consecutive layers … Continue reading

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Wednesday 06/29/11

9:40 A –3:30 P, 7:50 P – 9:20 P Finish ON confocal MP07Hz_snaD image 2.8Hz_snaD rescue for counting.  – still drift issue during acquisition as lens heats. Literature review: Set1 not Trx is major H3K4 trimethyltransferase (Lis) Trans-activation in mammalian … Continue reading

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Protected: sna rescue, shadow/distal removed, dramatically lowers expression

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Protected: Weaker effect on reporter when removing distal sna enhancer

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Protected: High mRNA expression upon proximal sna enhancer deletion

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Protected: bcd 1x v 6x cont

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