Tag Archives: making antibodies

Friday 06/08/12

Posted on June 8, 2012 by admin

10:00 -8:00P, 9:00P -11:20P Flip fly cages Finish clean up O/N STORM run.  Coopying files from Alistair5 to Alistair4. move in situ probe-test slides into boxes.  (Move archive boxes into 4C bin to make more space in 4C drawer?) 11:00 … Continue reading →

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Thursday 05/26/12

Posted on April 26, 2012 by admin

10:00 A – 11:45 P Check colonies from Bx-C round 1 cloning: all plates look good, no lawns. Gel-extract linearized Taf, Tub Gel-extract newly cloned Bx-C probes Antibody synthesis: antibodies 61-69 Flip collection cages: 10:15 A Washing out primary antibody … Continue reading →

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Thursday 03/15/12

Posted on March 15, 2012 by admin

9:45 A – 9:30P minor z drift issues still observed in STORM run. Time to take vertical beads for z-fit.  at 1:1000 density is a bit low. try 1:500 using ND 2-3 for z-fit bead images. Might be saturated?  Need … Continue reading →

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Sunday 02/05/12

Posted on February 5, 2012 by admin

9:40 A – 8:30P, 10:00P – 1:00A Flip fly plates try relabeling Dm0 antibodies with cy3B and 647 (first round labels still pretty concentrated protein.  Most of this is not antibody but we just reduce the dilution when adding to … Continue reading →

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Saturday 02/04/12

Posted on February 4, 2012 by admin

11:15 A – 7:30P Flip collection cages, 11:20 A Write JTB review Aliquot new dyes label new laminin Dm0 antibodies.  Protein conc very high, low labeling frequency.  see STORM antibody record. imaging failed.  had focus lock on wrong objective for … Continue reading →

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Wednesday 02/10/12

Posted on February 1, 2012 by admin

11:00A – 7:30P, 8:30P-11:30P Flip collection cages 11:20A Order Primers for Taf1, aTub84B, m13F/R Confocal: STORM prepped slides.  Both 1hr and 35 min samples are substantially over etched, most of sample is gone.  Try 20 min next (?). Confocal: MP08 … Continue reading →

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Friday 01/20/12

Posted on January 20, 2012 by admin

10:10 A -11:00 P labeling primary anti-histone antibodies (see Alistair_STORM_dyes log): pan-H3, H3K4me3, H3K27me3, and H3K27ac.  Need to filter Ac and K27 through column before binding.  Also filtered H3 and K4 — dilutes sample sufficiently to allow for multiple subsequent … Continue reading →

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