Friday 01/20/12

10:10 A -11:00 P

  • labeling primary anti-histone antibodies (see Alistair_STORM_dyes log): pan-H3, H3K4me3, H3K27me3, and H3K27ac. ¬†Need to filter Ac and K27 through column before binding. ¬†Also filtered H3 and K4 — dilutes sample sufficiently to allow for multiple subsequent labeling reactions from the same initial tube of antibody.
  • freeze MP08 large collection cages
  • test images of in situs
  • s02_enBio_WGA-touDig-EPcDig

    s02_enBio_WGA-touDig-EPcDig

    WGA-EpcDig-touDig

    WGA-EpcDig-touDig

    s04_insitus13_Ftz_H3K4_old

    s04_insitus13_Ftz_H3K4_old

    insitu13genes-WGA_ftz_H3K4

    insitu13genes-WGA_ftz_H3K4

    s01_WGA-en-dig_tou-bio

    s01_WGA-en-dig_tou-bio

  • O/N image at 40x of 28 MP08 embryos (rescues and esg/LacZ controls)
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