Author Archives: admin

9:30 A – 11:30 P (working remotely) Preparing slides for SDB Writing progress report for DR

9:40 A – 11:30 P STORM Focus lock got confused on cell #2, essentially all of the O/N run on yellow = lost. 2 modest bubbles also entered the chamber. maybe we need to dissolve the extra salts off with … Continue reading →

10:20 A – 11:50 P Coding Fixed bug in ReadDaxBeta (missing factor of 2 in converting bits and bytes). wrote script to make a movie of ‘building a storm image’ (PlotDax2Bin.m). Currently in AlistairBeta. Presentation prep for SDB working on … Continue reading →

8:00 A – 7:30 P, 8:3 0P – 11:50 P Presentation Finalizing talk for Wu group meeting most interested in large scale structure suggestion: hox-gene loci in mouse ES cells (larger regions easier to get structure) how about the Duboule … Continue reading →

11:00 A – 8:30 P, 10:00 P – 12:00 A STORM Analysis New Hal direction flip means TIRF beam aims to the right, not down!! All overnight images contain dramatic bleaching of sample subsequent frames ruined! STORM imaging set up … Continue reading →

11:00 – 5:30P Project 2 see notes OligoPaint library code Stupid transcriptome libraries necessarily have multiple copies of each gene for all the splice form variants. OligoArray returns these all as hits, even when their genome coordinates overlap. finished BLAST … Continue reading →

10:30 A – 11:30 PM Project 2 see notes Probe condition optimization correctly identified sample types STORM laser powers may not be optimal, see observations under STORM HPLC HPLC training with Bryan STORM Finish O/N STORM2 run of BX-C BB3 … Continue reading →