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Thursday 08/27/15

9:30 am – 7:00 pm, Lab meeting prep working on slides for Group B presentation, 9:30 am – 1:30 pm Two-color data analysis interesting results from ANT-C G6 quantification median overlap ~25%, median entanglement ~0.06 need to make figures still … Continue reading

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Wednesday 08/26/19

9:30 am – 9:00 pm Goals ChromatinCropper2 two color data for ANT-C G6 Spot finding for two color BX-C F6 data ChromatinCropper2 two color data tape station of sequencing prepped library for Ph-KD and control qPCR of sequencing prepped library … Continue reading

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Tuesday 08/25/15

9:30 am – 10:15 pm STORM finish overnight run switching still looks decent setup data to analyze on MORGAN. updated z-calibrations for both 647 and 750 parameters fitting bead data for drift correction all jobs launched before 1pm (probably need … Continue reading

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Monday 08/24/15

9:30 am – 5:15 pm, 8:20 pm – 11:30 pm Ph-polymerization Manuscript to do STORM section, reply to notes in main text Finish Layout Supp figure 2 and Supp figure 4 added supplement captions form both Check the legend to … Continue reading

Posted in Summaries | Comments Off on Monday 08/24/15

Friday 08/21/15

9:30 am – 7:00 pm Ph project rereading and revising manuscript sent comments back to team revising layout of Fig 5. revising response to reviewers sent updates back to team STORM imaging Jeff realigned lasers on STORM4 table, fixing the … Continue reading

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Thursday 08/20/15

9:30 am – 1:30 am Microscope troubleshooting might be able to try 2-color imaging in STORM5 with George E and shutters. doesn’t work. No emission filter to block 405 2 color conventional images of my stains look great though. trying … Continue reading

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Wednesday 08/19/15

9:00 am – 11:30 pm Visiting postdoc candidate interviews presentation 11 – 1:30 pm lunch 1:30 – 2:00 pm individual meeting 4-4:30 pm STORM finish imaging 2 color take bead images for z-calibration take bead images for chromatic correction Chromatin … Continue reading

Posted in Summaries | Comments Off on Wednesday 08/19/15

Tuesday 08/18/15

9:45 am – 8:00 pm, remotely, 9:15 pm – 11:00 pm RNAi validation Finish library prep run final bead purification and elution background prep contact Bauer core to confirm dates for tape analyzer reading background for KAPA_Library_Quantification stresses don’t freeze … Continue reading

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Monday 08/17/15

9:45 am RNA library prep setup protocol from NEB discuss plans with Jeff request training on BioAnalyzer and/or TapeStation instrument for library QC collecting kit components RNA purification run out 1 uL on a 1% agarose gel in TAE treat … Continue reading

Posted in Summaries | Comments Off on Monday 08/17/15