Author Archives: admin

coding

Polishing MERFISH code for public release. Plan take 140 gene library, create short fasta of just complete genes, run it through Launch OligoArray add parse OligoArray (as a function) into this script add assemble secondaries and target regions into as … Continue reading

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Wednesday 02/03/16

10:00 am – 5:00 pm Probe synthesis start synthesizing 2kb probes (with SW secondaries and Fast secondaries) running T7 reaction O/N. To do test SDS treatment of embryos test 2 kb probes (scope time on Friday evening). Results from yesterday … Continue reading

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Thursday 02/04/16

12:00 pm – 6:30 pm (10:00 am – 12:00 pm working remotely on library). Library prep updated 15 kb en library to use F primer L6F5 with L6R2 instead of F1, which I already used in lib7 ordered en 15 … Continue reading

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Tuesday 02/02/16

8:45 am – 10:40 pm schedule Rinse out probes, set up primary hybe for bit 1 for embryo staining experiment tracing BX-C Wu lab group meeting meeting with Scolnick at Broad Garner seminar on academic advice (see protected notes) continuing … Continue reading

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Protected: Dan Norcea: running a lab

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Protected: Ethan: Packing your parachute: Career Advice

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Monday 02/01/16

9:05 am – 7:30 pm, 8:15 pm – 11:40 pm Imaging testing control L4E24 embryos. stains look great. compare to L7 embryos — no staining. ran series removing S2 with toe-hold 5 bp. Looks excellent. Troubleshooting Gel tests try to … Continue reading

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Sunday, 01/31/16

6:00 pm – 7:00 pm Embryo staining just going for labeling — set heat block to 100C spec new library: label just new library, 3 uL in 20 uL buffer. control embryo, restain Friday’s failed sample with L4E24 P2/S2

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Protected: Lab Meeting 01/29/16

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Friday 01/29/16

9:00 am – 7:15 pm Meetings lab meeting (see notes) embryo staining & imaging stained BX-C locus with Bit 1 (9:00 am) rinsed out stain broke coverslip 1 after it detached from the plastic petri dish before the the nail … Continue reading

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