Category Archives: Fly Work

Saturday 08/16/14

5:30 pm – 12:10 am Data analysis Analyzing recent multi-color chromatin data with ChromatinCropper.fig not dramatically different overlap/exclusion between yellow-yellow and yellow-black yellow-black is however a ‘closer’ boundary (at least in the black-to-yellow direction) since the black domain is more … Continue reading

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Wednesday 02/20/13

10:00 A – 7:15P, 8:30P – 11:15P STORM Samples: BXC cy5 (single primary) and BXC cy5 + 750 secondary BXC-cy5 mounted for imaging 12:00P Realigned optics Taking bead calibration images Working out parameters to do z-scan. Can’t go too far … Continue reading

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Saturday 02/16/13

10:30 A – 8:30P, 10:00P – 11:55P STORM Analysis DaoSTORM analysis still running on Fab7-A647 Pc-750 Read write steps from USB connected hard-drive very slow when this analysis is running. (I’m not sure how much slower the analysis goes to … Continue reading

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Monday 02/11/13

9:30 A – 7:15, 10:00P – 11:45P Postdoc candidate see lab meeting notes Fly Work Flip collection cage 9:30A. O/N collection poor, not worth fixing. good collection at 1P, should probably flip around 1:30 and fix later. Collect AM virgins … Continue reading

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Thursday 02/07/13

9:30 AM – 8:30P STORM Finish O/N STORM imaging Pc-488 not bleaching well. Maybe 750 will be better. Fly Work Esc virgins emerging — collecting. some non-Sp (Df/esc[2] — it’s okay). No males yet to score phenotype AM collection 2x … Continue reading

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Monday 01/28/13

9:45A – 10:00 P Lab meeting (notes) Fly work scored 5 more back-cross lines (no rescues still):  [spreadsheet 0AjSqkxgziU1YdENuMzFjejM0TnNXUEQ1OWdHRW1OY1E 602 202 sheet = 1] Flipped and watered bottle cross of Df/CyO,esc[2] x Sp/esc[6] males. New bottle cross of Df/CyO,esc[2] x Sp/esc[6] males … Continue reading

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Saturday 01/05/12

11:00A – 3:30P, 4:30P – 10:45 P collect virgins DB Sp/CyoZ; TM2/TM6 MTD sna[18]/CyoZ/+; TM3/+/Pr esc[6]/CyO Df/CyO,esc[2] – not needed (need to cross esc[6] to Sp/CyO first). E(spl)[D] sim[D] collect Tub,Hu non-ebony male potential recombinants from 20+ lines.  Cross to … Continue reading

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Tuesday 12/11/12

10:10 A Check PCRs (in blocks 3A (sim screening) and 3B (Espl screening) better way to do this: complementation test back to original sim[D] and Espl[D] lines.  Just need to expand those lines back to bottles, will need ~100 vials … Continue reading

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Wednesday 11/22/12

9:10 A — 8:00P, 10:30P – 12:45 A UltaCryo collect prepped samples 10A – 2P, cutting ultra cryo sections of 1-10hr MTD DFISH-fixed embryos For next time: denser embryo packing in smaller gelatin sections Cryo protect at least 30 min … Continue reading

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Monday 11/19/12

9:15 A — 7:15 P, 8:15P – 1:15 A Cell labeling hot washes for probe first slide (2x at 60C) 1x at RT remove and save primary antibodies at RT for primary first slide. PBT rinses (3x soln change, 3rd … Continue reading

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