Saturday 01/05/12

11:00A – 3:30P, 4:30P – 10:45 P

• collect virgins
  • DB Sp/CyoZ; TM2/TM6
  • MTD
  • sna[18]/CyoZ/+; TM3/+/Pr
  • esc[6]/CyO
  • Df/CyO,esc[2] – not needed (need to cross esc[6] to Sp/CyO first).
  • E(spl)[D]
  • sim[D]
• collect Tub,Hu non-ebony male potential recombinants from 20+ lines.  Cross to sim[D] and Espl[D] virgins.  Keep track of tube of origin for each.
• Planning for MTD x shRNA crosses at 29C
• order mat-alpha Tub driver + daugtherless deletion + dorsal deletion + twi mutant.
  • 5531: da[10]/inv(2RL)-Gla  (4 kb x-ray deletion)
  • 7838: dorsal-deletion 100kb.  w¹¹¹⁸; Df(2L)Exel7068/CyO
  • 2381: twi[1]/CyO.  cn¹ twi¹ bw¹ sp¹/CyO
  • 3299: cn¹ sna¹⁸ twi³ bw¹ sp¹/CyO
  • 7062: mat-alpha Gal4-VP16 on 2
  • 7063: mat-alpha Gal4-VP16 on 3

Confocal

• O/N MP08 sna[D] (most recent staining) on 780.  Focus on cc13 embryos (found 6)
• O/N MP06Hz, focus on cc13 embryos (13) on 700.

Literature — (see Mendeley log)