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lab meeting 01/28/13
Will, BPS practice talk ISWI remodlers can assemble arrays denov. space nucleosomes out regularly — critical for silencing and folding leading existing model: loop propagation DNA binding domain scoops in 10 bp from entry side (feeds this into entry side). … Continue reading
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Sunday 09/02/12
11:30 A – 3:00P, 5:30P-8:00P writing to mounia about sna ths getting data for mounia on sna ThsPr: cc14l, cc13 writing to Mike / Jacques / Mounia about papers m116a & b has a bit high false detection rate (higher … Continue reading
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Tagged coding, communication, image analysis
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Saturday 05/12/12
11:30 A – 5:30P Finish DR acceptance statement / bio etc. Literature review: Shandilya transcription review, evidence for transcription factories: Xu 2008 (minichromosomes), Osborne 2004 (DNA / RNA FISH comparison) working on 3D vector bead alignment code
Sunday 02/05/12
9:40 A – 8:30P, 10:00P – 1:00A Flip fly plates try relabeling Dm0 antibodies with cy3B and 647 (first round labels still pretty concentrated protein. Most of this is not antibody but we just reduce the dilution when adding to … Continue reading
Thursday 10/20/11
10:00 A – 11:30 P Working on GPU analysis (see post) test PCR of en BAC working on manuscript figure 7: documenting ‘domino effect’ in MP08 embryos. — should really stain and image more embryos. learning Insight3 with Yari — … Continue reading
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Love and Fear in the Lab with Uri Alon
Why don’t we discuss the social side? Scientific values: objective and rational — “what we do” (or tell ourselves). don’t have language to discuss subjective emotional side. — excitement. Subjective component: skills: patience, persistence, listening skills, mentoring skills — start simple … Continue reading
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Monday 07/25/11
9:00 A – 7:15 P, 8:00 P – 9:45 P Playing with fonts and table layout for website — export from Word .htm doesn’t work well, export from OpenOffice is simpler but not all elements work for pasting into wordpress … Continue reading
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Tuesday 05/03/11
9:00 A – 7:00P, 7:40P – 10:00P Finish confocal run (C55, 22C). Save data imviewer filter on C55_30C data. Request from ML: Movie: Pol II sitting in groove getting regularly timed, quantile release events from SEC / Mediator interactions. Stochastic … Continue reading
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Genomic mapping
non-unique NM# to CG# table creates conflicts. Try again mapping everything to CG labeled genome.
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Genomic mapping
Galaxy thinks gd7 fastq all have low quality scores (1 to 4 on a scale of -5 to 40). Mapping .gff gd7 reads to browser to check quality. Looks good on UCSC browser, except that squish display doesn’t work. Taking … Continue reading
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