Tag Archives: image analysis

Thursday 10/18/12

9:40 A – 7:00P, 8:20P – 10:45P Flip collection cage, 9:45A.  Keep plate to make new bottles Pick up more 10x TAE and 20x SSC from stock room Picked up 6 new pints of 100% EtOH. Found issue with K27:H3 … Continue reading

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Sunday 10/14/12

10:20 A Fly work New cage of MTD (not crossed, just as control).  Use for Pc and Su(Z)12 staining.  Methanol treat after primaries to improve in situ. DNA FISH Day 3: DNA-FISH + antibody. [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE sheet=8 602 302] postfix 1 … Continue reading

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Wednesday 10/10/12

10:00 A – 8:00P, 9:00P – 11:15P Collect virgins Working on presentation for meeting with Xiaowei (see notes below on data processing and analysis). Meeting with XZ.  Notes here. Processing STORM data 8/25/12 AbdA, no 750 movies analyzed yet in … Continue reading

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Sunday 09/16/12

1:00P – 7:00P, 9:00P-10:30P Analyzing data from DaoSTORM run.  InsightM crashed all 750 movies, so unable to compare at the moment.  However the DaoSTORM images look good and qualitatively similar to previous analyses done with insight. Modifying movie2vectorSTORM to deal … Continue reading

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Sunday 09/02/12

11:30 A – 3:00P, 5:30P-8:00P writing to mounia about sna ths getting data for mounia on sna ThsPr: cc14l, cc13 writing to Mike / Jacques / Mounia about papers m116a & b has a bit high false detection rate (higher … Continue reading

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Saturday 09/01/12

4:45 P – 7:00P Break down STORM4 O/N run on S1 AbdA AbdB Ubx Break down confocal O/N run of sog Pr. Analyzing snail Pr Ths Pr data. Fixed bug in snail_comp_promoters code: index i was being used both in … Continue reading

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Wednesday 08/28/12

9:50 A-7:00P, 8:30P – 11:50P pick up drive from HCBI STORM4 lost focus O/N.  Reset focus, continue imaging. Trying to get good matches for pnrPr dynamics in simulation, not just end states. Dyes not switching well in 15 min etched … Continue reading

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Tuesday 08/28/12

9:30 A – 7:00PM, 7:45P – 12:00A 20 min etch Pc-647 sample.  Turnkey inspection looks over-etched, most embryos quite sparse. Forgot that previous MP07 samples have Draq5 not DAPI.   Extracting nuclear masks manually through inverting DAPI channel and using … Continue reading

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Saturday 08/25/12

10:30 A – 7:30P, 8:3oP – 10:40P check O/N confocal run, 13 embryos 2 channels 70 sections on speed 5 started at 8P, 12/13 finished. (several embryos were not substantially flattened, hence the extra sections). expansion of lens has changed … Continue reading

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Friday 08/24/12

10:15 A – 9:30P running analysis on images sent by Mounia.   see post4160. Write up snail analysis updates (see post). Trying to fix lsm format error on image 13 and later of m106a.  Unable to ID parse error. Write … Continue reading

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