Tag Archives: planning

Protected: project 2 03/19/14

Posted on March 18, 2014 by admin

There is no excerpt because this is a protected post.

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Monday 04/01/13

Posted on April 1, 2013 by admin

10:00 A – 7:00P, 9:00P-10:00P General hp psc1200 printer not printing color correctly. lab meeting (notes) journal club (notes) Review writing Insight 1: enhancer switching between obstructed and unobstructed case is the bursting model with different tx rates from ‘off’ … Continue reading →

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oligopaint probe making planning

Posted on April 1, 2013 by admin

emPCR ready libraries: AbdA AbdB Ubx en-locus 100kb Black 100kb Yellow 100kb Green 100kb Synthesis schemes: Teritaries + Nicking PCR using extended primers = Forward primer (21bp) + Secondary binding site (32 bp) Nick off R primer Denaturing PAGE Gel … Continue reading →

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Research Planning 12/14/12

Posted on December 15, 2012 by admin

Use antibody accessability as a proxy for PolII accessability. Compare levels of staining of H3K27me3 in intact vs. mechanically spread nuclei.  (First we need to see if H3K27me3 even remains associated in spread nuclei.  Try spreading nuclei using the ‘shearing’ … Continue reading →

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Saturday 11/10/12

Posted on November 10, 2012 by admin

10:45 A – 8:00P Snail project stuff Collect virgin Pr/Tm3Z, Sp/CyOZ, and TM2/TM6 (males + virgins) Order primers for screening E(spl) and sim[D].  Also for en and inv introns DNA FISH probe design Wrote new matlab script for concatinating text … Continue reading →

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Friday 11/09/12

Posted on November 9, 2012 by admin

9:30 A – 8:30P Making double mutants Collect virgin TM2/TM6 flies Clear Pr/TM3,hbZ and Sp/CyOZ for virgin collection to remake double balanced 2,3 flies. TM6b = AntpHue1 . TM2 = Ubx130esemc2 . E(spl) and sim[D](7646) both have TM6b, Tb.  Should be lethal with TM6. … Continue reading →

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Wednesday 10/31/12

Posted on October 31, 2012 by admin

9:45 A – 8:40P, 9:45P-11:45P Submit revised snail kinetics paper. Collect virgin Espl/sim cross to TM2/TM6 Collect virgin Act5 Gal4.  Cleared and added new bottle of these to virign collection. washout Pc antibody from O/N MTD post fix 5 min … Continue reading →

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Protected: Experiments to try later

Posted on October 18, 2012 by admin

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Getting Funding for BIRS workshop

Posted on October 2, 2012 by admin

Plan going forward with BIRS funding applications Mary to look into NSF application Rob to look into ICAM application Andrew to look into INTELBIOMAT Alistair to investigate NIH application Links: http://grants2.nih.gov/grants/funding/r13/index.htm http://grants.nih.gov/grants/funding/424/SF424_RR_Guide_General_Adobe_VerB.pdf http://grants.nih.gov/grants/guide/pa-files/PA-12-212.html#_Section_IV._Application_1 http://grants.nih.gov/grants/guide/pa-files/PA-12-212.html http://www.nsf.gov/pubs/gpg/nsf04_23/2.jsp#IID7      

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Friday 09/21/12

Posted on September 21, 2012 by admin

(Working remotely from Berkeley) Ideas: can I have the analysis script call Git bash and update the code with a ‘run to export images’ (check with Carl?) export a text file at the same time as it writes an image: … Continue reading →

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