oligopaint probe making planning
emPCR ready libraries:
- AbdA
- AbdB
- Ubx
- en-locus 100kb
- Black 100kb
- Yellow 100kb
- Green 100kb
Synthesis schemes:
Teritaries + Nicking
- PCR using extended primers = Forward primer (21bp) + Secondary binding site (32 bp)
- Nick off R primer
- Denaturing PAGE Gel extract
Tertiaries + Exonuclease
- Start with same PCR + extended primers
- Second PCR using PPT-primer in place of extended primer
- Exonuclease digestion
- Denaturing PAGE Gel extract
Assymetric PCR
- Start with same PCR + extended primers
- Second PCR using just extended F primers, no reverse. 40 rounds?
- Run on PAGE